| Lithium prevents colchicine-induced apoptosis in rat cerebellar granule neurons. | |
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MedLine Citation:
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PMID: 15005753 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Here we evaluated the neuroprotective effects of two well-known mood stabilizers, lithium and valproic acid (VPA), against colchicine neurotoxicity in cerebellar granule cells (CGNs). METHODS: The CGNs were differentiated for 7 days, pretreated with lithium or VPA for 24 h and after colchicine 1 microM was added. Cellular damage was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) method and apoptosis in CGNs was characterized by chromatin condensation and DNA fragmentation. RESULTS: Incubation with lithium (1-5 mM) attenuated this apoptosis markedly, in a dose-dependent way however, the addition of VPA (0.5-2 mM) did not protect CGNs. Colchicine-induced apoptosis is mediated through the activation of caspase-3. An increase in caspase-3 activity was detected within 18 h and was blocked in presence of lithium 5 mM. CONCLUSIONS: Our data indicate that lithium treatment is selectively neuroprotective; however, in our experimental conditions VPA did not protect CGNs from apoptosis induced by colchicine. Our results support the hypothesis that distinct pathways mediate the neuroprotective effects of lithium and VPA. |
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Authors:
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E G Jordà; E Verdaguer; A Morano; A Jiménez; A M Canudas; A Camins; M Pallàs |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bipolar disorders Volume: 6 ISSN: 1398-5647 ISO Abbreviation: Bipolar Disord Publication Date: 2004 Apr |
Date Detail:
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Created Date: 2004-03-09 Completed Date: 2004-07-15 Revised Date: 2007-03-22 |
Medline Journal Info:
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Nlm Unique ID: 100883596 Medline TA: Bipolar Disord Country: Denmark |
Other Details:
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Languages: eng Pagination: 144-9 Citation Subset: IM |
Affiliation:
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Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Nucli Universitari de Pedralbes, Barcelona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Cerebellum / drug effects*, pathology* Colchicine / administration & dosage, antagonists & inhibitors*, pharmacology* Colorimetry Flow Cytometry Gout Suppressants / administration & dosage, pharmacology* Lithium Carbonate / administration & dosage, pharmacology* Neurons / drug effects*, pathology* Neuroprotective Agents / administration & dosage, pharmacology* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Gout Suppressants; 0/Neuroprotective Agents; 554-13-2/Lithium Carbonate; 64-86-8/Colchicine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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