| Lithium inhibits cell volume regulation by acting on chloride channels and modifies ultrastructures of the cell membrane in nasopharyngeal carcinoma cells. | |
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MedLine Citation:
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PMID: 20522412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lithium salts have been used as a class of mood stabilizing agents to treat bipolar disorders for over a century. Although lithium is known to affect cell proliferation, apoptosis and migration, the underlying mechanisms have not been well-explored. Emerging evidence indicates that cell volume regulation and volume-activated chloride channels are involved in cell proliferation, apoptosis and migration. To understand the mechanism of lithium's actions, we investigated the effect of lithium chloride on cell volume regulation and volume-activated chloride channels in the nasopharyngeal carcinoma cell line CNE-2Z. Our results show that lithium chloride attenuates regulatory volume decrease induced by 47% hypotonic challenges in a concentration-dependent manner with an IC(50) of 756 microM. Using the patch clamp techniques, we further show that lithium chloride concentration-dependently (IC(50)=440 microM) inhibits the volume-activated chloride current as well as the background chloride current. Furthermore, using a nanoscale atomic force microscope, we show that lithium chloride prevents the hypotonic challenge-induced changes in the ultrastructures of the cell membrane. These changes include an increase in the number and the size of the small holes, which are observed in the surface of the cell membrane under isotonic conditions. The lithium chloride-induced inhibition in cell volume regulation, in volume-activated chloride current and in the ultrastructures of the cell membrane may contribute to its effects on cell proliferation, apoptosis and migration. |
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Authors:
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Zhiquan Bai; Haifeng Zhang; Linyan Zhu; Wanhong Zuo; Jianghong Ye; Jianqiang Feng; Liwei Wang; Lixin Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-27 |
Journal Detail:
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Title: European journal of pharmacology Volume: 641 ISSN: 1879-0712 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-15 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 88-95 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Physiology, Medical College, Jinan University, Guangzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects Carcinoma / metabolism, pathology Cell Line, Tumor Cell Movement / drug effects Cell Proliferation / drug effects Cell Size / drug effects* Chloride Channels / antagonists & inhibitors, metabolism*, physiology Chlorides / metabolism, pharmacology Dose-Response Relationship, Drug Erythrocyte Membrane / metabolism, ultrastructure Humans Inhibitory Concentration 50 Lithium Compounds / therapeutic use* Microscopy, Atomic Force Nasopharyngeal Neoplasms / metabolism*, pathology* Patch-Clamp Techniques |
| Chemical | |
Reg. No./Substance:
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0/Chloride Channels; 0/Chlorides; 0/Lithium Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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