| Lithium chloride regulates connexin43 in skeletal myoblasts in vitro: possible involvement in Wnt/beta-catenin signaling. | |
| | |
MedLine Citation:
|
PMID: 18972237 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Gap junction channels composed of connexin43 (Cx43) are essential for normal myogenic differentiation and skeletal muscle regeneration. Here, the aim was to study whether lithium chloride (LiCl) could regulate Cx43 expression and gap junction channel function by mimicking the Wnt/beta-catenin pathway in primary myoblasts. Cx43 mRNA expression in myoblasts was up-regulated in response to 5 mM LiCl. The enhanced Cx43 protein expression resulting from treatment with 5 and 10 mM LiCl for 24 h increased gap-junctional coupling in myoblasts. However, no obvious changes were observed with 20 mM LiCl. Furthermore, chronic treatment with 10 mM LiCl decreased Cx43 protein expression compared with untreated cells. The authors showed that LiCl mimicked the active canonical Wnt/beta-catenin signaling by glycogen synthase kinase-3beta (GSK-3beta) inactivation and accumulation of the effector protein beta-catenin into the nucleus. These results suggest that LiCl regulates Cx43 expression in skeletal myoblasts in vitro partly by a Wnt/beta-catenin-dependent pathway. |
| | |
Authors:
|
W J Du; J K Li; Q Y Wang; J B Hou; B Yu |
Related Documents
:
|
16520547 - Regulation of wnt signaling by protein-protein interaction and post-translational modif... 11169847 - Immunohistochemical expression of smads 1-6 in the 15-day gestation mouse embryo: signa... 17634137 - Clusterin expression can be modulated by changes in tcf1-mediated wnt signaling. 15203747 - The significance of the wnt pathway in the pathology of human cancers. 23222817 - Cathepsin b and upar regulate self-renewal of glioma-initiating cells through gli-regul... 8706937 - Differential post-transcriptional control of ornithine decarboxylase and spermidine-spe... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Cell communication & adhesion Volume: 15 ISSN: 1543-5180 ISO Abbreviation: Cell Commun. Adhes. Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-11-03 Completed Date: 2008-12-31 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101096596 Medline TA: Cell Commun Adhes Country: United States |
Other Details:
|
Languages: eng Pagination: 261-71 Citation Subset: IM |
Affiliation:
|
Department of Cardiology, The Second Clinical College of Harbin Medical University, Harbin, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Cell Nucleus / metabolism Cells, Cultured Connexin 43 / drug effects*, genetics Dose-Response Relationship, Drug Lithium Chloride / pharmacology* Microscopy, Confocal Muscle, Skeletal / cytology, metabolism* Myoblasts / metabolism* RNA, Messenger / biosynthesis Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Up-Regulation / drug effects Wnt Proteins / metabolism* beta Catenin / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Catnb protein, rat; 0/Connexin 43; 0/RNA, Messenger; 0/Wnt Proteins; 0/beta Catenin; 7447-41-8/Lithium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A comparison of the merits of using birth weight, or appropriateness of birth weight to gestational ...
Next Document: Simplified culture techniques for growth and differentiation of murine and human pre-adipocytes for ...