| Listeriolysin O secretion by Listeria monocytogenes in the presence of cysteine and sorbate. | |
| | |
MedLine Citation:
|
PMID: 7766229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The influence of cysteine or cysteine-HCl and their combination with potassium sorbate on growth of Listeria monocytogenes and listeriolysin O (LLO) secretion, and on activation of LLO in the haemolysin assay system was studied. Both cysteine and cysteine-HCl (10 and 20 mmol l-1) enhanced LLO secretion in tryptic soy broth supplemented with yeast extract during 24 h incubation at 35 degrees C. While sorbate did not affect growth, it suppressed both LLO secretion and LLO activation by cysteine in the haemolysin activity assay. These findings provide further evidence that sulphydryl-containing enzymes are implicated in the mechanism of microbial inhibition of sorbate. Addition of sorbate to foods has the potential of inactivating listeriolysin and other thiol-dependent toxins. |
| | |
Authors:
|
Y Kouassi; L A Shelef |
Related Documents
:
|
20206119 - Detection of femtomole quantities of mature cathepsin k with zymography. 6762209 - A cysteine proteinase secreted from human breast tumours is immunologically related to ... 2139119 - Cathepsins b, h and l activities in gingival crevicular fluid from chronic adult period... 2140339 - Inhibition of streptococcus mutans glucosyltransferase activity by antiserum to a subse... 10543449 - Fluorometric microassays for the determination of cathepsin l and cathepsin s activitie... 1812719 - Proteolytic activation of human procathepsin d. 9951139 - Trichomonas tenax proteolytic activity. 1546949 - Purification and some properties of alcohol oxidase from alkane-grown candida tropicalis. 12951239 - A detailed kinetic study of mox-1, a plasmid-encoded class c beta-lactamase. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Letters in applied microbiology Volume: 20 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 1995 May |
Date Detail:
|
Created Date: 1995-06-13 Completed Date: 1995-06-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 295-9 Citation Subset: B |
Affiliation:
|
Department of Nutrition and Food Science, Wayne State University, Detroit, MI, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bacterial Toxins* Cysteine / pharmacology* Erythrocytes Heat-Shock Proteins / drug effects, secretion* Hemolysin Proteins Hemolysis / physiology Listeria monocytogenes / drug effects*, growth & development, metabolism Sheep Sorbic Acid / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Toxins; 0/Heat-Shock Proteins; 0/Hemolysin Proteins; 110-44-1/Sorbic Acid; 52-90-4/Cysteine; 72270-41-8/hlyA protein, Listeria monocytogenes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of plasmid profiling as a typing method for epidemiologically related Clostridium perfringens is...
Next Document: Evaluation of a new chromogenic agar medium for the identification of urinary tract pathogens.