| Listeriolysin O enhances cytoplasmic delivery by Her-2 targeting liposomes. | |
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MedLine Citation:
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PMID: 20201742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To enhance cytoplasmic delivery of liposomal contents to breast cancer cells, the authors have attached the pore-forming protein, listeriolysin O (LLO), to thermosensitive liposomes. The antibody trastuzumab (Herceptin) was also conjugated with the outer surface of the liposomes, resulting in highly specific binding and internalization into mammary epithelial cells that overexpress the human epidermal growth factor receptor 2 (Her-2). The liposomes were preloaded with a marker fluorescent dye, and the effect of LLO on the distribution of dye within the cells was monitored using fluorescence microscopy. Owing to the thermosensitive nature of the liposomes, hyperthermia at 42 degrees C triggered the release of the encapsulated fluorescent calcein from the endocytosed liposomes into the interior of the endosomes. LLO, when conjugated to these liposomes, subsequently formed pores in the endosomal membrane, allowing calcein to flow out of the endosomal compartment into the cytoplasm. Her-2-targeted liposomes bearing LLO delivered a 22-fold greater concentration of calcein to mammary epithelial cells that overexpress Her-2 compared to cells with normal Her-2 expression. Thus, the addition of LLO to preformed liposomes offers a method for significantly enhancing delivery of liposomal contents to the cytoplasm of targeted cells. |
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Authors:
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Max Kullberg; Jesse Lee Owens; Kristine Mann |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of drug targeting Volume: 18 ISSN: 1029-2330 ISO Abbreviation: J Drug Target Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-08-10 Revised Date: 2011-04-18 |
Medline Journal Info:
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Nlm Unique ID: 9312476 Medline TA: J Drug Target Country: England |
Other Details:
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Languages: eng Pagination: 313-20 Citation Subset: IM |
Affiliation:
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Biomedical Program, University of Alaska, Anchorage, Alaska 99508, USA. afmpk@uaa.alaska.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Toxins
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administration & dosage* Cell Line Cytoplasm / metabolism* Fluorescent Dyes Heat-Shock Proteins / administration & dosage* Hemolysin Proteins / administration & dosage* Humans Liposomes* Microscopy, Fluorescence Receptor, erbB-2 / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Toxins; 0/Fluorescent Dyes; 0/Heat-Shock Proteins; 0/Hemolysin Proteins; 0/Liposomes; 72270-41-8/hlyA protein, Listeria monocytogenes; EC 2.7.10.1/Receptor, erbB-2 |
| Comments/Corrections | |
Comment In:
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Nanomedicine (Lond). 2011 Feb;6(2):314 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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