Document Detail


Listeria monocytogenes attachment to and detachment from stainless steel surfaces in a simulated dairy processing environment.
MedLine Citation:
PMID:  19767476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence of pathogens in dairy products is often associated with contamination via bacteria attached to food-processing equipment, especially from areas where cleaning/sanitation is difficult. In this study, the attachment of Listeria monocytogenes on stainless steel (SS), followed by detachment and growth in foods, was evaluated under conditions simulating a dairy processing environment. Initially, SS coupons were immersed in milk, vanilla custard, and yogurt inoculated with the pathogen (10(7) CFU/ml or CFU/g) and incubated at two temperatures (5 and 20 degrees C) for 7 days. By the end of incubation, cells were mechanically detached from coupons and used to inoculate freshly pasteurized milk which was subsequently stored at 5 degrees C for 20 days. The suspended cells in all three products in which SS coupons were immersed were also used to inoculate freshly pasteurized milk (5 degrees C for 20 days). When SS coupons were immersed in milk, shorter lag phases were obtained for detached than for planktonically grown cells, regardless of the preincubation temperature (5 or 20 degrees C). The opposite was observed when custard incubated at 20 degrees C was used to prepare the two types of inocula. However, in this case, a significant increase in growth rate was also evident when the inoculum was derived from detached cells. In another parallel study, while L. monocytogenes was not detectable on SS coupons after 7 days of incubation (at 5 degrees C) in inoculated yogurt, marked detachment and growth were observed when these coupons were subsequently transferred and incubated at 5 degrees C in fresh milk or/and custard. Overall, the results obtained extend our knowledge on the risk related to contamination of dairy products with detached L. monocytogenes cells.
Authors:
Sofia Poimenidou; Charalambia A Belessi; Efstathios D Giaouris; Antonia S Gounadaki; George-John E Nychas; Panagiotis N Skandamis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-18
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  75     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-09     Completed Date:  2010-03-25     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7182-8     Citation Subset:  IM    
Affiliation:
Laboratory of Food Quality Control and Hygiene, Department of Food Science and Technology, Agricultural University of Athens, Athens 11855, Greece.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion
Cold Temperature
Colony Count, Microbial
Dairy Products / microbiology
Dairying / instrumentation*,  methods
Equipment Contamination*
Food Handling / instrumentation*,  methods
Food Microbiology*
Listeria monocytogenes / growth & development*
Milk / microbiology
Stainless Steel*
Time Factors
Yogurt / microbiology
Chemical
Reg. No./Substance:
12597-68-1/Stainless Steel
Comments/Corrections

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