Document Detail

Listeria monocytogenes PrsA2 is required for virulence factor secretion and bacterial viability within the host cell cytosol.
MedLine Citation:
PMID:  20823208     Owner:  NLM     Status:  MEDLINE    
In the course of establishing its replication niche within the cytosol of infected host cells, the facultative intracellular bacterial pathogen Listeria monocytogenes must efficiently regulate the secretion and activity of multiple virulence factors. L. monocytogenes encodes two predicted posttranslocation secretion chaperones, PrsA1 and PrsA2, and evidence suggests that PrsA2 has been specifically adapted for bacterial pathogenesis. PrsA-like chaperones have been identified in a number of Gram-positive bacteria, where they are reported to function at the bacterial membrane-cell wall interface to assist in the folding of proteins translocated across the membrane; in some cases, these proteins have been found to be essential for bacterial viability. In this study, the contributions of PrsA2 and PrsA1 to L. monocytogenes growth and protein secretion were investigated in vitro and in vivo. Neither PrsA2 nor PrsA1 was found to be essential for L. monocytogenes growth in broth culture; however, optimal bacterial viability was found to be dependent upon PrsA2 for L. monocytogenes located within the cytosol of host cells. Proteomic analyses of prsA2 mutant strains in the presence of a mutationally activated allele of the virulence regulator PrfA revealed a critical requirement for PrsA2 activity under conditions of PrfA activation, an event which normally takes place within the host cell cytosol. Despite a high degree of amino acid similarity, no detectable degree of functional overlap was observed between PrsA2 and PrsA1. Our results indicate a critical requirement for PrsA2 under conditions relevant to host cell infection.
Francis Alonzo; Nancy E Freitag
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-09-07
Journal Detail:
Title:  Infection and immunity     Volume:  78     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-19     Completed Date:  2010-11-12     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4944-57     Citation Subset:  IM    
Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612-7344, USA.
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MeSH Terms
Bacterial Proteins
Cell Line
Cytosol / microbiology*
Fibroblasts / microbiology
Gene Expression Regulation, Bacterial
Listeria monocytogenes / genetics,  growth & development,  pathogenicity*
Listeriosis / microbiology*
Macrophages / microbiology
Microbial Viability*
Peptidylprolyl Isomerase / genetics,  metabolism*
Virulence Factors / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/Virulence Factors; EC Isomerase; EC protein, Listeria monocytogenes

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