Document Detail


Listeria monocytogenes efficiently invades Caco-2 cells after low-temperature storage in broth and on deli meat.
MedLine Citation:
PMID:  20443727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to investigate how various growth conditions influence the virulence of Listeria monocytogenes monitored by its ability to invade the epithelial cell lines Caco-2 and INT-407. The growth conditions examined were modified atmosphere-packaged deli meat and brain heart infusion broth (BHI) with and without salt. Five strains of L. monocytogenes were selected to investigate their invasiveness and all strains invaded Caco-2 cells at higher levels than INT-407 cells. Further, the clinical strains (3443 and 3734) were more invasive (p < 0.05) than the strains isolated from meat and food-processing environments (3008, 3126, and 4140) after grown in BHI at 30 degrees C. This attenuation could not be ascribed to a defective Internalin A as all strains encoded an intact inlA gene. To determine the influence of food products on virulence, the ability of L. monocytogenes to invade Caco-2 cells was compared after growth on a fermented sausage and on cured cooked ham to that of bacteria grown in BHI broth supplemented with salt. Samples were stored under chilling conditions for up to 4 weeks. The results showed no difference (p > 0.05) in invasiveness after 7 days at 10 degrees C in BHI broth or on sausage, whereas a slight increase (p < 0.05) was observed after incubation on ham for 2 and 4 weeks compared to that in BHI broth. Most importantly, our results show that L. monocytogenes efficiently invade Caco-2 cells even after 4 weeks of storage at chilled temperature. This is highly relevant for safety assessment of this organism in food as these conditions reflect storage of ready-to-eat food products in domestic refrigerators.
Authors:
Marianne Halberg Larsen; Anette Granly Koch; Hanne Ingmer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  7     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-02     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1013-8     Citation Subset:  IM    
Affiliation:
Department of Veterinary Disease Biology, Centre for Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark. mhl@life.ku.dk
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics
Bacteriological Techniques
Caco-2 Cells
Cell Line
Cold Temperature
Culture Media
Epithelial Cells / microbiology
Food Handling
Food Microbiology
Humans
Intestines / microbiology
Listeria monocytogenes / genetics,  growth & development*,  pathogenicity*
Listeriosis / microbiology
Meat / microbiology
Meat Products / microbiology
Species Specificity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 139948-71-3/internalin protein, Bacteria

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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