Document Detail

Listeria monocytogenes Can Form Biofilms in Tap Water and Enter Into the Viable but Non-Cultivable State.
MedLine Citation:
PMID:  24452996     Owner:  NLM     Status:  Publisher    
Listeria monocytogenes is a foodborne pathogen that can be transmitted through contaminated raw food or by ready-to-eat products that have been in contact with contaminated surfaces. Tap water (TW) is used to wash produce, as a processed food constituent and to wash processing surfaces and floors. The main aim of this work was to investigate the formation and survival of L. monocytogenes biofilms on stainless steel (SS) coupons in TW at 4, 22, 30 and 37 °C. For that, coupons with biofilm were visualised in situ while other coupons were scraped to quantify total cells by SYTO 9, cultivable numbers by plating onto brain heart infusion agar and viable numbers by the direct viable count method. Results showed that L. monocytogenes can form biofilms on SS surfaces in TW at any temperature, including at 4 °C. The number of total cells was similar for all the conditions tested while cultivable numbers varied between the level of detection (<8.3 CFU cm(-2)) and 3.5 × 10(5) CFU cm(-2), meaning between 7.0 × 10(4) and 1.1 × 10(7) cells cm(-2) have entered the viable but non-cultivable (VBNC) state. This work clearly demonstrates that L. monocytogenes can form biofilms in TW and that sessile cells can remain viable and cultivable in some conditions for at least the 48 h investigated. On the other hand, VBNC adaptation suggests that the pathogen can remain undetectable using traditional culture recovery techniques, which may give a false indication of processing surface hygiene status, leading to potential cross-contamination of food products.
Maria S Gião; Charles W Keevil
Related Documents :
19462326 - No effect on intake and liking of soup enhanced with mono-sodium glutamate and celery p...
12691176 - The evolution of the control of food intake.
24560666 - Novel synergistic approach to exploit the bactericidal efficacy of commercial disinfect...
21983276 - Toxic impact of two organophosphate insecticides on biochemical parameters of a food fi...
7014326 - Regulation of food intake and body weight in insulin.
19325706 - Early fasting is long lasting: differences in early nutritional conditions reappear und...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-23
Journal Detail:
Title:  Microbial ecology     Volume:  -     ISSN:  1432-184X     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Phase II Trial of Adjuvant Oral Thalidomide Following Cytoreductive Surgery and Hyperthermic Intrape...
Next Document:  Antidepressant Drugs and the Risk of Suicide in Children and Adolescents.