Document Detail


Liquid chromatography/electrospray tandem mass spectrometry characterization of styrene metabolism in man and in rat.
MedLine Citation:
PMID:  12478566     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liquid chromatography with electrospray tandem mass spectrometry was used to characterize the metabolism of styrene in man and in rat. To improve identification and characterization of minor styrene metabolites, rats were co-exposed to styrene and styrene-d(8). In addition to the main styrene metabolites, mandelic acid and phenylglyoxylic acid, and specific mercapturic acids, phenylhydroxyethylmercapturic acids (PHEMAs), other minor metabolites, including phenylglycine, N-acetyl-S-(phenacyl)cysteine, 4-vinylphenol and styreneglycol conjugates (glucuronides and sulfates) were identified and determined both in human and rat urine. Phenylglycine and N-acetyl-S-(phenacyl)cysteine have been hypothesized to occur, but never detected in human or rat urine after styrene exposure. 4-Vinylphenol and styrene glycol had already been recognized as styrene metabolites, but never determined as intact glucuronide and sulfate conjugates. Failure to identify 1- and 2-phenylethanol conjugates suggests that phenylethanol might be an intermediate metabolite, but it is not a conjugated catabolite. A method for the simultaneous determination of mandelic acid, phenylglyoxylic acid, phenyglycine and the four PHEMA diastereoisomers has been developed and validated. For those glucuronide and sulfate conjugates whose standards are not commercially available, a method for semiquantitative analysis, based on the use of structurally similar compounds as standards, has been developed. This approach was found to be valid for the determination of 4-vinylphenol glucuronide and 4-vinylphenol sulfate.
Authors:
Paola Manini; Roberta Andreoli; Diana Poli; Giuseppe De Palma; Antonio Mutti; Wilfried M A Niessen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  16     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2002  
Date Detail:
Created Date:  2002-12-12     Completed Date:  2003-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2239-48     Citation Subset:  IM    
Copyright Information:
Copyright 2002 John Wiley & Sons, Ltd.
Affiliation:
Dipartimento di Clinica Medica, Nefrologia e Scienze della Prevenzione, Laboratorio di Tossicologia Industriale, Università degli Studi di Parma, Via Gramsci 14, 43100 Parma, Italy. paola.manini@unipr.it
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / urine
Animals
Chromatography, Liquid
Glycine / urine
Humans
Mass Spectrometry / methods*
Molecular Structure
Occupational Exposure
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Styrene / administration & dosage,  metabolism*,  pharmacokinetics,  urine*
Chemical
Reg. No./Substance:
100-42-5/Styrene; 56-40-6/Glycine; 616-91-1/Acetylcysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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