| Liquid chromatographic/mass spectrometric investigation on the reaction products in the peroxidase-catalyzed oxidation of o-phenylenediamine by hydrogen peroxide. | |
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MedLine Citation:
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PMID: 15900478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mass spectrometric evidence was obtained to confirm that the main reaction product of the horseradish peroxidase (POD)-catalyzed oxidation of o-phenylenediamine (OPD) by hydrogen peroxide is 2,3-diaminophenazine. Although this reaction is one of the most widespread detection schemes in enzyme-linked immunosorbent assays (ELISAs), the literature data on the identity of the reaction product(s) have been strongly contradictory throughout the last few decades. Liquid chromatography with UV/Vis and mass spectrometric detection as well as exact mass measurements after LC fraction collection have led to the unambiguous identification of 2,3-diaminophenazine as main reaction product. 2,2'-Diaminoazobenzene, which is frequently described in other publications to be the major reaction product, was not detected at all. |
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Authors:
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Christel Hempen; Suze M van Leeuwen; Heinrich Luftmann; Uwe Karst |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-04-21 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 382 ISSN: 1618-2642 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-05-18 Completed Date: 2006-06-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 234-8 Citation Subset: IM |
Affiliation:
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Chemical Analysis Group and MESA Institute for Nanotechnology, University of Twente, PO Box 217, 7500, AE Enschede, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Catalysis Chromatography, Liquid / methods* Enzyme-Linked Immunosorbent Assay Hydrogen Peroxide / chemistry* Oxidation-Reduction Phenylenediamines / chemistry* Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Phenylenediamines; 7722-84-1/Hydrogen Peroxide; 95-54-5/1,2-diaminobenzene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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