| Liquid chromatography/tandem mass spectrometry assay for the quantification of troxerutin in human plasma. | |
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MedLine Citation:
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PMID: 17072901 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, rapid, sensitive and specific liquid chromatography/tandem mass spectrometry method was developed and validated to quantify troxerutin in human plasma. The analyte and rutin, used as the internal standard, were analyzed on a Phenomenex Synergi Fusion RP column interfaced with a triple-quadrupole tandem mass spectrometer using positive electrospray ionization. Acetonitrile/water (20:80 v/v) was used as the isocratic mobile phase, with 0.1% formic acid in water. A simple sample preparation method of protein precipitation with perchloric acid was employed. The assay was linear over the concentration range 31.25-4000 pg/mL. Correlation coefficients generated by linear regression with a 1/x(2) weighting factor ranged from 0.9991 to 0.9996. The intra- and inter-day precision over the entire concentration range were less than 12.28%. The method was successfully applied to a pharmacokinetic study after oral administration of a 300 mg troxerutin drop pill to 18 healthy volunteers. |
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Authors:
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Fei Liu; Yu Xu; Lei Rui; Shu Gao; Haijun Dong; Qingxiang Guo |
Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 20 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-11-20 Completed Date: 2007-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 3522-6 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2006 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Chemistry, University of Science and Technology of China, Hefei 230026, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood Chemical Analysis / methods* Chromatography, Liquid / methods* Humans Hydroxyethylrutoside / analogs & derivatives*, blood, pharmacokinetics Male Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization / methods* Therapeutic Equivalency |
| Chemical | |
Reg. No./Substance:
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0/Hydroxyethylrutoside; 7085-55-4/troxerutin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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