Document Detail


Liquid chromatography method for determination of mefenamic acid in human serum.
MedLine Citation:
PMID:  14698254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple, rapid and specific method for analysis of mefenamic acid (I) in serum by a sensitive high-performance liquid chromatography is described. Only 70 microl of serum and a little sample work-up is required. A simple procedure of extraction by dichloromethane followed by evaporation to dryness under gentle stream of nitrogen and dissolving the dried residue in mobile phase was used. The mefenamic acid peak was separated from endogenous peaks on a C(8) column by a mobile phase of acetonitrile-water (50:50, v/v, pH 3). Mefenamic acid and internal standard (IS) (diclofenac) were eluted at 7.4 and 5.4 min, respectively. The limit of quantitation of mefenamic acid in serum was 25 ng/ml at 280 nm. The method was linear over the range of 25-2000 ng/ml with r(2) of 0.998. Mean recovery for mefenamic acid was 110%.
Authors:
Mohammad-Reza Rouini; Ali Asadipour; Yalda Hoseinzadeh Ardakani; Fakhredin Aghdasi
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  800     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2003-12-30     Completed Date:  2004-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-92     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451, Iran. rouini@sina.tums.ac.ir
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / blood*
Biological Availability
Calibration
Chromatography, High Pressure Liquid
Cross-Over Studies
Double-Blind Method
Humans
Indicators and Reagents
Mefenamic Acid / blood*,  pharmacokinetics
Reference Standards
Reproducibility of Results
Therapeutic Equivalency
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Indicators and Reagents; 61-68-7/Mefenamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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