| Liquid chromatography method for determination of mefenamic acid in human serum. | |
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MedLine Citation:
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PMID: 14698254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, rapid and specific method for analysis of mefenamic acid (I) in serum by a sensitive high-performance liquid chromatography is described. Only 70 microl of serum and a little sample work-up is required. A simple procedure of extraction by dichloromethane followed by evaporation to dryness under gentle stream of nitrogen and dissolving the dried residue in mobile phase was used. The mefenamic acid peak was separated from endogenous peaks on a C(8) column by a mobile phase of acetonitrile-water (50:50, v/v, pH 3). Mefenamic acid and internal standard (IS) (diclofenac) were eluted at 7.4 and 5.4 min, respectively. The limit of quantitation of mefenamic acid in serum was 25 ng/ml at 280 nm. The method was linear over the range of 25-2000 ng/ml with r(2) of 0.998. Mean recovery for mefenamic acid was 110%. |
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Authors:
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Mohammad-Reza Rouini; Ali Asadipour; Yalda Hoseinzadeh Ardakani; Fakhredin Aghdasi |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 800 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2003-12-30 Completed Date: 2004-08-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: United States |
Other Details:
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Languages: eng Pagination: 189-92 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451, Iran. rouini@sina.tums.ac.ir |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Inflammatory Agents, Non-Steroidal
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blood* Biological Availability Calibration Chromatography, High Pressure Liquid Cross-Over Studies Double-Blind Method Humans Indicators and Reagents Mefenamic Acid / blood*, pharmacokinetics Reference Standards Reproducibility of Results Therapeutic Equivalency |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Indicators and Reagents; 61-68-7/Mefenamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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