Document Detail


Liquid chromatography and mass spectrometry in food allergen detection.
MedLine Citation:
PMID:  21333155     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Food allergy is an important issue in the field of food safety because of the hazards for affected persons and the hygiene requirements and legal regulations imposed on the food industry. Consumer protection and law enforcement require suitable analytical techniques for the detection of allergens in foods. Immunological methods are currently preferred; however, confirmatory alternatives are needed. The determination of allergenic proteins by liquid chromatography and mass spectrometry has greatly advanced in recent years, and gel-free allergenomics is becoming a routinely used approach for the identification and quantitation of food allergens. The present review provides a brief overview of the principles of proteomic procedures, various chromatographic set ups, and mass spectrometry instrumentation used in allergenomics. A compendium of published liquid chromatography methods, proteomic analyses, typical marker peptides, and quantitative assays for 14 main allergy-causing foods is also included.
Authors:
Christiane Kruse Fæste; Helene Thorsen Rønning; Uwe Christians; Per Einar Granum
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  74     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  316-45     Citation Subset:  IM    
Affiliation:
Section of Chemistry, Department of Feed and Food Safety, National Veterinary Institute, P.O. Box 750 Sentrum, Oslo N-0106, Norway; Centre for Food Safety, Norwegian School of Veterinary Science, Oslo 0033, Norway. christiane.faste@vetinst.no.
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