Document Detail


Liquid chromatographic procedure for the determination of novobiocin residues in bovine milk: interlaboratory study.
MedLine Citation:
PMID:  7703728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Novobiocin is used for the treatment of mastitis in dairy cattle. In 1982, the tolerance was set at 0.1 ppm in milk from dairy animals. A laboratory trial has been completed for a liquid chromatographic procedure that can quantitate novobiocin residues in bovine milk at tolerance level. In this procedure the milk is diluted with buffer, the proteins are precipitated with methanol, and the solution is filtered. Novobiocin is determined after separation of milk components using reversed-phase chromatography with UV detection at 340 nm. The participating laboratories analyzed 2 concentrations of biologically incurred residues as well as control milk and control milk fortified at 0.05, 0.1, and 0.2 ppm. Recoveries of novobiocin reported by the participating laboratories were 89 to 99% at 0.05 ppm; 93 to 101% at 0.1 ppm; and 89 to 100% at 0.2 ppm. Coefficients of variation (CVs) ranged from 2.0 to 6.2%. The average concentrations for the low levels of incurred novobiocin in milk samples were 0.073, 0.072, and 0.081 ppm, with intralaboratory CVs of 3.3, 7.2, and 2.4%, respectively. The samples with high levels of incurred novobiocin averaged 0.139, 0.121, and 0.144 ppm, with CVs of 6.2, 0.7, and 4.7%, respectively.
Authors:
V B Reeves
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  78     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1995 Jan-Feb
Date Detail:
Created Date:  1995-05-11     Completed Date:  1995-05-11     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  55-8     Citation Subset:  IM    
Affiliation:
U.S. Food and Drug Administration, Center for Veterinary Medicine, Agricultural Research Center-East, Beltsville, MD 20705.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Liquid
Drug Residues / analysis*
Indicators and Reagents
Milk / chemistry*
Novobiocin / analysis*
Spectrophotometry, Ultraviolet
United States
United States Food and Drug Administration
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 303-81-1/Novobiocin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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