Document Detail


Liquid chromatographic determination of tocopherols and tocotrienols in margarine, infant foods, and vegetables.
MedLine Citation:
PMID:  8757449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A liquid chromatographic (LC) method was developed for determination of tocopherols and tocotrienols in foods. Tocopherols and tocotrienols were released after saponification of test portions for 40 min at 80 degrees C, followed by extraction with hexane-ethyl acetate. After evaporation of the organic solvents, the extract was injected in the LC system. Separation of individual tocopherols and tocotrienols was satisfactory. Some interferences were encountered from tocomoneols, tocodienols, 2-tert-butyl-4-hydroxyanisole (BHA), 2,6-di-tert-butyl-4-methylphenol, (BHT), and plastochromanol-8. The response of the LC system was linear over a range of 0-10 micrograms/mL for tocopherols and tocotrienols. The detection limit for these compounds was 0.1 micrograms/mL. Repeatabilty relative standard deviation values for tocopherols and tocotrienols in margarine, infant formula, and broccoli (concentration range, 0.11-22 mg/100 g) varied from 1.3 to 6.4%. Recoveries ranged from 91 to 105%.
Authors:
E J Konings; H H Roomans; P R Beljaars
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  79     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1996 Jul-Aug
Date Detail:
Created Date:  1997-02-14     Completed Date:  1997-02-14     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  902-6     Citation Subset:  IM    
Affiliation:
Inspectorate for Health Protection, Food Inspection Service, Maastricht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / analysis*,  metabolism
Chromans / analysis,  metabolism
Chromatography, Liquid
Food Additives
Food Analysis*
Freeze Drying
Humans
Infant Food / analysis*
Infant, Newborn
Margarine / analysis*
Reproducibility of Results
Vegetables / chemistry*
Vitamin E / analogs & derivatives,  analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Chromans; 0/Food Additives; 1406-18-4/Vitamin E; 1721-51-3/tocotrienol, alpha; 25612-59-3/tocotrienol, delta; 4382-43-8/plastochromanol 8; 490-23-3/tocotrienol, beta; 8029-82-1/Margarine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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