| Liquid chromatographic determination of gamma-aminobutyric acid in cerebrospinal fluid using 2-hydroxynaphthaldehyde as derivatizing reagent. | |
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MedLine Citation:
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PMID: 12705983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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gamma Aminobutyric acid (GABA) was determined by precolumn derivatization with 2-hydroxynaphthaldehyde and elution was made using Phenomenex C(18), 5 microm column with methanol: water (62:38 v/v) and UV detection at 330 nm. In a mixture containing glycine, l-lysine and tyramine GABA separated completely. A number of amines and amino acids tested did not affect the response of GABA. A linear calibration curve was obtained for GABA in the range of 1.2-28.0 microg/ml with detection limit of 2.8 ng/injection (5 microl). The method was used for the determination of GABA in cerebral spinal fluid (CSF) samples and gave results of 19.0 to 22.4 microg/m1 with coefficient of variation 2.4% |
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Authors:
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M Y Khuhawar; A D Rajper |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 788 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-04-22 Completed Date: 2004-03-08 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: United States |
Other Details:
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Languages: eng Pagination: 413-8 Citation Subset: IM |
Affiliation:
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Dr. M.A. Kazi Institute of Chemistry, University of Sindh Jamshoro, Sindh, Pakistan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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chemistry* Calibration Chromatography, High Pressure Liquid / methods* Humans Indicators and Reagents / chemistry* Naphthalenes / chemistry* Sensitivity and Specificity Spectrophotometry, Ultraviolet gamma-Aminobutyric Acid / cerebrospinal fluid* |
| Chemical | |
Reg. No./Substance:
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0/2-hydroxynaphthaldehyde; 0/Aldehydes; 0/Indicators and Reagents; 0/Naphthalenes; 56-12-2/gamma-Aminobutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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