Document Detail


Liquid chromatographic analysis of vitamin K1 in milk-based infant formula with matrix solid-phase dispersion.
MedLine Citation:
PMID:  10513014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A liquid chromatographic method for vitamin K1 in milk-based infant formula is described. The vitamins are extracted from infant formula by matrix solid-phase dispersion and quantitated by reversed-phase chromatography with fluorescence detection. Vitamin K1 is converted to the fluorescent hydroquinone with a postcolumn zinc reductive reactor. The limit of detection is 12 pg, and the limit of quantitation is 38 pg on-column. Linear responses were obtained in the range 0.55-22.1 ng/ml (r2 = 0.9998). Recoveries of vitamin K1 from an analyte-fortified blank material for milk-based infant formula averaged 91.7% (n = 25). The method provides a rapid, specific, and easily controlled assay for vitamin K1 in fortified infant formula.
Authors:
G W Chase; R R Eitenmiller; A R Long
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  82     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1999 Sep-Oct
Date Detail:
Created Date:  1999-11-26     Completed Date:  1999-11-26     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1140-5     Citation Subset:  IM    
Affiliation:
U.S. Food and Drug Administration, Southeast Regional Laboratory, Atlanta, GA 30309, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Liquid*
Infant Food / analysis*
Linear Models
Milk / chemistry*
Sensitivity and Specificity
Time Factors
Vitamin K 1 / analysis*
Chemical
Reg. No./Substance:
84-80-0/Vitamin K 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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