| Liquid Chromatography-Mass Spectrometric Multiple Reaction Monitoring-based Strategies for Expanding Targeted Profiling towards Quantitative Metabolomics. | |
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MedLine Citation:
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PMID: 22519369 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Recent advances in analytical methodologies have made it possible to bring the metabolomic profiling into the field of quantitative metabolomics that permits precise measurements of comprehensive small-molecule profiles within a biological system. Modern liquid chromatography-tandem mass spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM) mode serves as the foundation for accurate simultaneous multi-analyte quantitation across large sample sets to provide high-quality and valuable information on target molecular profiles in complex systems. Despite the intrinsic multiplexing potential of the LC-MRM-MS technique, the key bottleneck in current LC-MRM-based assays is generally the limited analyte coverage and throughput capacity. Nowadays, the MRM-based approach has emerged as an attractive strategy for quantitative proteomic analysis and high-throughput biomarker discovery. So far, the full potential of the contemporary LC-MRM methodology unleashed for quantitative metabolite profiling and metabolomic measurements of non-petidic small molecules is rarely discussed. In this review we attempt to provide an overview on the major recent developments in LC-MRM-based strategies for quantitative profiling of multi- and non-target small molecules in biological samples. This article highlights the utility and power of the LC-MRM-based targeted approaches as valuable bioanalytical tools for low-cost, multiplexed quantitation on a large scale, with special emphasis on the promise of combining various strategies for expanding coverage and throughput of the LC-MRM-based assays with multivariate statistical methods to cover the gap between a widely targeted profiling and the conventional large-scale unknown screening towards comparative or quantitative metabolomics. General issues raised in metabolite profiling, such as current aspects of bioanalysis, methodological dilemmas and basic challenges in quantitative metabolomics are addressed, and different strategies to circumvent the existing bottleneck and potential pitfalls of the conventional LC-MRM-MS techniques are outlined and proposed. In addition, a brief description of the rudiments of LC-MRM-MS and its recent applications in combination with such strategies for biomarker quantitation and verification is also described. |
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Authors:
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Bin Guo; Bo Chen; Aiming Liu; Weitao Zhu; Shouzhuo Yao |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-18 |
Journal Detail:
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Title: Current drug metabolism Volume: - ISSN: 1875-5453 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100960533 Medline TA: Curr Drug Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education of China, Hunan Normal University, 36 Lushan Road, Changsha 410081, China. binnguo@126.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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