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Lipovitellin is a Non-Self Recognition Receptor with Opsonic Activity.
MedLine Citation:
PMID:  20857311     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Lipovitellin (Lv), a glycolipoprotein, is a major component of the egg yolk, which is usually regarded as an energy reserve of nutrients essential for growth and development. We have purified Lv from ovulated eggs of the rosy barb Puntius conchonius by two-step chromatography and characterized it by staining with periodic acid/Schiff reagent and Sudan black B, amino acid composition analysis, and peptide mass fingerprinting. The results of ligand and bacterial binding assays, an enzyme-linked immunosorbent assay (ELISA), and the phagocytosis test revealed, for the first time, that the purified native form of P. conchonius Lv acts as a pattern recognition molecule with multiple specificities capable of identifying pathogen-associated molecular patterns (PAMPs), including those of lipopolysaccharide, lipoteichoic acid, and peptidoglycan, rather than self components and that it can bind Gram-negative and -positive bacteria, such as Escherichia coli and Staphylococcus aureus. These tests also showed that the P. conchonius Lv functions as an opsonin capable of enhancing macrophage phagocytosis. Taken together, these characteristics suggest that in developing embryos/larvae of P. conchonius, the native form of Lv may be physiologically involved in the sensing of invading pathogens via interaction with PAMPs and in the recruitment of the primitive macrophages that appear in early embryos to phagocytose and digest the pathogens, thereby protecting them from pathogenic attacks.
Authors:
Jun Zhang; Shicui Zhang
Publication Detail:
Type:  Journal Article     Date:  2010-09-21
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  13     ISSN:  1436-2236     ISO Abbreviation:  Mar. Biotechnol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  441-50     Citation Subset:  IM    
Affiliation:
Department of Marine Biology and Laboratory for Evolution and Development, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.
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