| Is Lipotoxicity presents in the early stages of an experimental model of autoimmune diabetes? Further studies in the multiple low dose of streptozotocin model. | |
| | |
MedLine Citation:
|
PMID: 21099313 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
An increased availability of plasma free fatty acids (FFA) seems to play a role in the early stages of experimental type 1 diabetes mellitus induced in C57BL/6J mice by multiple low doses of streptozotoxin (mld-STZ). We analyzed the temporal changes of: (1) plasma and skeletal muscle lipids and their relationship with glucose metabolism; (2) triglyceride (Tg) concentration in isolated islets; (3) intraperitoneal glucose tolerance test; and (4) insulin secretion patterns when the three mutually interactive glucose signaling pathways were activated. Animals were killed by cervical dislocation at days 4, 6, 7, 8, 9 and 12 post first injection of mld-STZ. Compared with control mice, we observed: (1) at day 6, a significant increase of plasma FFA and both muscle and islet Tg content and a significant decrease of muscle pyruvate dehydrogenase activity. These parameters further deteriorated with time. (2) plasma Tg, glucose and insulin levels and glucose tolerance test were significantly different only after day 8. (3) an increase in both phases of the glucose plus palmitate-stimulated insulin secretion was observed at day 4. This effect progressively decreased since day 7 up to day 9. Moreover, an inhibitory action of cerulenin over glucose plus palmitate-stimulated insulin secretion was observed between days 6 and 9. Taken together these results suggest that early alteration in carbohydrate and lipid metabolism could represent a "metabolic window" which would develop between days 6 and 8. Afterwards, subsequent immunological alterations, apoptosis and necrosis induced the destruction of β cells and would mask the results mentioned above. |
| | |
Authors:
|
Liliana Karabatas; Maria Eugenia Oliva; Eduardo Dascal; Gustavo J Hein; Claudia Pastorale; Adriana Chicco; Yolanda B Lombardo; Juan C Basabe |
Related Documents
:
|
6364843 - Hyperinsulinemia and in vivo very-low-density lipoprotein-triglyceride kinetics. 18558553 - Effects of nicotinamide supplementation on liver and serum contents of amino acids in d... 16026323 - Role for poly(adp-ribose) polymerase activation in diabetic nephropathy, neuropathy and... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Islets Volume: 2 ISSN: 1938-2022 ISO Abbreviation: Islets Publication Date: 2010 May-Jun |
Date Detail:
|
Created Date: 2010-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101495366 Medline TA: Islets Country: United States |
Other Details:
|
Languages: eng Pagination: 190-9 Citation Subset: IM |
Affiliation:
|
Endocrinology Research Center (CEDIE), Hospital Ricardo Gutierrez, Buenos Aires, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ? 1-antitrypsin enhances insulin secretion and prevents cytokine-mediated apoptosis in pancreatic ?-...
Next Document: Serine protease inhibitors suppress pancreatic endogenous proteases and modulate bacterial neutral p...