Document Detail


Lipoteichoic acids from Streptococcus sanguis.
MedLine Citation:
PMID:  4208135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two lipoteichoic acids, membrane (MLTA) and wall (WLTA), have been purified from Streptococcus sanguis by Sepharose and Ecteola-cellulose column chromatographies and concanavalin A-conjugated Sepharose affinity column chromatography. The teichoic acids were homogenous as judged by disc gel electrophoresis, column chromatography, and double diffusion tests. Both MLTA and WLTA consisted of glycerol, phosphate, glucose, and fatty acids in the ratios of 0.95:1:0.71:0.046 and 0.99:1:0.79:0.023, respectively. alpha-Glycerol-phosphate was obtained by the partial acid hydrolysis of the lipoteichoic acids suggesting that their backbone structure consists of the glycerol moieties linked by 1, 3-phosphodiester bonds. Both WLTA and MLTA form aggregates, perhaps due to micelle formation, in concentrated solution. The aggregate form of MLTA dissociates to a much greater extent than that of WLTA under similar conditions.
Authors:
T H Chiu; L I Emdur; D Platt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  118     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 May 
Date Detail:
Created Date:  1974-07-16     Completed Date:  1974-07-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  471-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / analysis
Cell Wall / analysis
Chromatography
Chromatography, Affinity
Chromatography, Gas
Chromatography, Paper
Concanavalin A
Electrophoresis, Disc
Fatty Acids / analysis
Glucose / analysis
Glycerol / analysis
Glycerophosphates / analysis
Immunodiffusion
Lipids / analysis*,  isolation & purification
Phosphates / analysis
Streptococcus / analysis*,  cytology
Surface-Active Agents
Teichoic Acids / analysis*,  isolation & purification
Tritium
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Glycerophosphates; 0/Lipids; 0/Phosphates; 0/Surface-Active Agents; 0/Teichoic Acids; 10028-17-8/Tritium; 11028-71-0/Concanavalin A; 50-99-7/Glucose; 56-81-5/Glycerol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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