Document Detail


Lipoteichoic acid induces delayed myocardial protection in isolated rat hearts: a comparison with endotoxin.
MedLine Citation:
PMID:  18635308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Preconditioning with bacterial wall fragments lipopolysaccharide (LPS) or lipoteichoic acid (LTA) reduce myocardial infarct size after ischaemia and reperfusion (I/R) in rats. Preconditioning with LTA reduces neutrophil accumulation during reperfusion and thereby ameliorates one of myocardial reperfusion injury's most important mechanisms. In this study, we use an ex vivo model of regional myocardial I/R to investigate LTA versus LPS induced preconditioning in a system devoid of leukocytes. METHODS: Rats were subjected to LTA or LPS with or without dexamethasone pre-treatment. Twenty-four hours after LTA or LPS challenge, hearts were removed and retrogradely perfused in a Langendorff set-up. Hearts underwent 20 min of regional ischaemia followed by 2 h of reperfusion. Ischaemic preconditioning (IPC) was performed as a positive control. Myocardial infarct size was determined as the primary end-point. RESULTS: LTA and LPS preconditioning both lead to a marked reduction in infarct size similar to IPC, however, no significant differences were found between LTA and LPS. The reduction in infarct size was abrogated by dexamethasone pre-treatment. CONCLUSION: We conclude that preconditioning with LTA and likewise with LPS confers myocardial protection in an ex vivo setting devoid of leukocytes. Dexamethasone inhibits preconditioning, suggesting that the underlying mechanism is dependent upon induction of a systemic inflammatory response to a LTA or LPS stimulus.
Authors:
Patrick Schober; Guenther Oprea; Jan Mersmann; Antje Nebert; Kai Zacharowski; Paula A Zacharowski
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-07-17
Journal Detail:
Title:  Resuscitation     Volume:  79     ISSN:  0300-9572     ISO Abbreviation:  Resuscitation     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-27     Completed Date:  2009-02-10     Revised Date:  2009-08-25    
Medline Journal Info:
Nlm Unique ID:  0332173     Medline TA:  Resuscitation     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  311-5     Citation Subset:  IM    
Affiliation:
Department of Anaesthesiology, VU University Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Inflammatory Agents / therapeutic use
Dexamethasone / therapeutic use
Disease Models, Animal
Endotoxins / therapeutic use*
Ischemic Preconditioning, Myocardial / methods*
Lipopolysaccharides / therapeutic use*
Male
Myocardial Infarction / pathology*,  prevention & control*
Neutrophils
Rats
Rats, Wistar
Systemic Inflammatory Response Syndrome / prevention & control
Teichoic Acids / therapeutic use*
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Endotoxins; 0/Lipopolysaccharides; 0/Teichoic Acids; 50-02-2/Dexamethasone; 56411-57-5/lipoteichoic acid; 67924-63-4/endotoxin, Escherichia coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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