| Liposomes enriched in oleic acid are less susceptible to oxidation and have less proinflammatory activity when exposed to oxidizing conditions. | |
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MedLine Citation:
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PMID: 9643355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As there is frequently a reciprocal relationship between oleic acid and linoleic acid content in LDL after dietary supplementation, it is difficult to determine the independent effects of oleic and linoleic acid on LDL oxidation. It is also unknown whether monounsaturated fatty acid enrichment might reduce the generation of proinflammatory products that occur when the polyunsaturated fatty acid-rich phospholipids within lipoproteins undergo mild oxidation. To address these issues, we exposed liposomes containing variable amounts of oleic, linoleic, and arachidonic acid to oxidizing conditions. Liposomes enriched in oleic acid but with constant amounts of linoleic acid were less susceptible to oxidation and had significantly greater lag times and time to half maximum conjugated diene formation. When mildly oxidized, liposomes containing either linoleic acid or arachadonic acid increased monocyte chemotaxis and monocyte adhesion to endothelial cells nearly 5-fold, demonstrating that oxidation products of both these polyunsaturated fatty acids are bioactive. The addition of a platelet activating factor receptor antagonist to endothelial cells inhibited stimulation of monocyte adhesion by oxidized liposomes, suggesting that some bioactive oxidation products of polyunsaturated fatty acids may resemble platelet activating factor in structure. In contrast, when liposomes were enriched in oleic acid, monocyte chemotaxis and monocyte adhesion were nearly completely inhibited. These results suggest that enriching lipoproteins with oleic acid may reduce oxidation both by a direct "antioxidant"-like effect and by reducing the amount of linoleic acid available for oxidation as well as reduce the generation of bioactive particles that occur during mild oxidation. |
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Authors:
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C Lee; J Barnett; P D Reaven |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of lipid research Volume: 39 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-08-28 Completed Date: 1998-08-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1239-47 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of California, San Diego, La Jolla 92093-0682, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonate 15-Lipoxygenase / metabolism Cell Adhesion / drug effects, physiology Cell Line Chemotaxis, Leukocyte / drug effects, physiology Fibroblasts / drug effects, physiology Kinetics Liposomes / chemistry*, pharmacology Macrophages, Peritoneal / drug effects, physiology* Mice Monocytes / drug effects, physiology* Oleic Acid / chemistry*, pharmacology* Oxidation-Reduction Phosphatidylcholines / chemistry*, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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HL-14197/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 0/Phosphatidylcholines; 112-80-1/Oleic Acid; EC 1.13.11.33/Arachidonate 15-Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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