Document Detail


Liposomes containing cationic dimethyl dioctadecyl ammonium bromide: formulation, quality control, and lipofection efficiency.
MedLine Citation:
PMID:  11839204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This article describes a novel, simple, and relatively inexpensive method to prepare cationic liposomes using an ethanol injection/pressure extrusion method. The study also demonstrated that binding erythrosine dye to cationic liposomes results in a shift of the absorption maximum of the dye from 528 nm to 549 nm at pH 4.25, allowing quantification and visualization of these vesicles. In addition, a relatively simple Ficoll-based gradient centrifugation method for separation of lipoplexes from unbound molecules is presented. Laboratory-formulated dimethyl dioctadecyl ammonium bromide (DDAB) containing liposomes were just as efficient in complexing nucleic acids as commercially available types, and binding increased as the positive to neutral lipid ratio was increased. Transfection efficiency of the DDAB-containing liposomes increased as the ratio of cationic to neutral lipid was increased from 1:1 to 4:1 with either PtdChol or DOPE as the neutral lipid. A concomitant increase in cytotoxicity of CSU-SA1 cancer cells was noted as the ratio of positive to neutral lipid of the liposomes was increased. Nevertheless, our present study showed that the 2:1 liposome is a good choice since it delivers functional plasmids at a comparable rate to commercial liposome formulations, has similar toxicities to the less harmful commercial liposomes, and is at least 1000-fold more economical to prepare inhouse, a major factor to be considered in preclinical and clinical studies with these carriers.
Authors:
Crispin R Dass; Todd L Walker; Mark A Burton
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Drug delivery     Volume:  9     ISSN:  1071-7544     ISO Abbreviation:  Drug Deliv     Publication Date:    2002 Jan-Mar
Date Detail:
Created Date:  2002-02-12     Completed Date:  2002-05-20     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  9417471     Medline TA:  Drug Deliv     Country:  England    
Other Details:
Languages:  eng     Pagination:  11-8     Citation Subset:  IM    
Affiliation:
Charles Sturt University, Wagga Wagga, Australia. cdass@medau.jnj.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cations
Cell Survival / drug effects
Chemistry, Pharmaceutical
Drug Carriers*
Fluorescent Dyes
Liposomes / chemistry*
Plasmids
Quaternary Ammonium Compounds / chemistry*,  pharmacology
Rats
Transfection
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Cations; 0/Drug Carriers; 0/Fluorescent Dyes; 0/Liposomes; 0/Quaternary Ammonium Compounds; 14357-21-2/dimethyldioctadecylammonium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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