| Liposomes and blood cells: a flow cytometric study. | |
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MedLine Citation:
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PMID: 14672416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To clarify the interactions of liposomes with blood cells, this study examined the behaviour of liposomes of a range of compositions in the presence of purified human blood cells in buffer or plasma; or in whole blood, or in mice in vivo. Liposomes, labeled with the hydrophilic fluorochrome, carboxy fluorescein (CF), or with membrane-sequestering R18 or FITC-labeled phospholipids, were mixed with blood cells and the appearance of the fluorochromes in the blood cell population was monitored by flow cytometry. Irrespective of composition, with or without poly(ethylene glycol), all types of liposomes were found to interact rapidly and dose-dependently with red cells, leukocytes and platelets, both in vitro and in vivo. This took place equally in the presence and the absence of plasma proteins and functional enzyme cascades, suggesting that the prime facie interaction is opsonization-independent and is consistent with liposome-blood cell fusion. |
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Authors:
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Iren Constantinescu; Elena Levin; Maria Gyongyossy-Issa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Artificial cells, blood substitutes, and immobilization biotechnology Volume: 31 ISSN: 1073-1199 ISO Abbreviation: Artif Cells Blood Substit Immobil Biotechnol Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-12-15 Completed Date: 2004-09-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9431307 Medline TA: Artif Cells Blood Substit Immobil Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 395-424 Citation Subset: IM |
Affiliation:
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Canadian Blood Services, Research & Development, c/o Department of Pathology, University of British Columbia, Vancouver, British Columbia, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals Blood Cells / metabolism* Blood Platelets / metabolism Buffers Drug Delivery Systems / methods* Flow Cytometry Fluorescent Dyes Humans Liposomes / chemistry*, pharmacokinetics* Mice Plasma |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Fluorescent Dyes; 0/Liposomes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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