Document Detail


Liposomes as immunological adjuvants in eliciting antibodies specific to the synthetic polypeptide poly(LTyr, LGlu)-poly(DLAla)--(LLys) with high frequency of site-associated idiotypic determinants.
MedLine Citation:
PMID:  7262180     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The antibody response to the synthetic polypeptide, poly(LTyr, LGlu)-poly(DLAla)--poly(LLys), [(T,G)-A--L], injected entrapped in liposomes which served as adjuvant has been analyzed. The liposomes used were composed of phosphatidylcholine, cholesterol, dicetylphosphate and DL alpha-tocopherol (molar ratios as 4:3:0.1:0.5) and therefore, were negatively charged. Since the (T,G)-A--L is also negatively charged, no free complexes were formed. The (T,G)-A--L was found to be entrapped inside the enclosed volume of the liposomes, and no (T,G)-A--L antigenic determinants could be detected on the liposomal membranes. Injection of high-responder C3H.SW (H-2b) mice with (T,G)-A--L-bearing liposomes demonstrated that the i.p. and the i.v. routes of immunization were efficient in eliciting (T, G)-A--L specific antibodies, whereas the i.d. injection led to poor antibody responses. The latter route of immunization is the most effective when (T,G)-A--L is injected in complete Freund's adjuvant (CFA). When low doses (0.1 and 1 microgram) of (T, G)-A--L were used for immunization, the liposomes were better adjuvants than CFA. The effectiveness of the liposomes as immunological adjuvants was also shown in their ability to induce high-potential, primed memory cells. The pattern of low (H-2k,a) and high (H-2b) responsiveness to (T,G)-A--L was retained following immunization with (T,G)-A--L entrapped in liposomes, as tested in two pairs of congenic strains. (T,G)-A--L-specific antibodies induced by injection with 1 microgram antigen entrapped in liposomes bear the (T,G)-A--L site-related idiotypic markers of C3H.SW (Igh-1a) mice in a significantly higher frequency than the homologous idiotypes, namely the antibodies elicited in this strain against (T,G)-A--L in CFA. Thus, liposomes may serve as adjuvants for the production of relatively restricted (T,G)-A--L-specific antibodies of high quality.
Authors:
R Lifshitz; C Gitler; E Mozes
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of immunology     Volume:  11     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1981 May 
Date Detail:
Created Date:  1981-10-29     Completed Date:  1981-10-29     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  398-404     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / pharmacology*
Animals
Antibody Formation
Antibody Specificity*
Binding Sites
Guinea Pigs
H-2 Antigens / genetics
Immunoglobulin Idiotypes
Isoelectric Focusing
Liposomes / immunology*
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Peptides / immunology
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/H-2 Antigens; 0/Immunoglobulin Idiotypes; 0/Liposomes; 0/Peptides; 28704-27-0/(T,G)-A-L

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