| Liposome delivery of quantum dots to the cytosol of live cells. | |
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MedLine Citation:
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PMID: 18572640 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An increasing number of studies have demonstrated the multiple advantages of using nanocrystals, such as Quantum dots, for biological imaging. Quantum dots functionalized with biomolecules on their surfaces were shown to be able to bind to specific extracellular targets via specific recognition and to be internalized inside the cells, thereby allowing the imaging of intracellular pathways. However, the use of Quantum dots for live tracking of intracellular molecules is relatively limited because of the difficulties encountered during the induction of Quantum dots across living cell membranes. In this study we show that cationic liposomes can deliver low concentrations of non-targeted Quantum dots into the cytosol of living cells via a lipid-mediated fusion with the cell membrane. The intracellular Quantum dots exhibit aggregation that appears dependent upon their concentration, but does not visibly affect cell viability. Our results point towards the use of cationic liposomes as an effective delivery system for targeted Quantum dots within the cell cytosol, which would facilitate live cell imaging of the labeled molecules. |
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Authors:
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Veronica Dudu; Melissa Ramcharan; M Lane Gilchrist; Eric C Holland; Maribel Vazquez |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of nanoscience and nanotechnology Volume: 8 ISSN: 1533-4880 ISO Abbreviation: J Nanosci Nanotechnol Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-06-24 Completed Date: 2008-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088195 Medline TA: J Nanosci Nanotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 2293-300 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, The City College of New York, 160 Convent Ave., T-402, New York, NY 10031, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cytosol
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metabolism* Hela Cells Humans Liposomes* Quantum Dots* |
| Grant Support | |
ID/Acronym/Agency:
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CA096299-09/CA/NCI NIH HHS; GM071703/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Liposomes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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