| Liposomal entrapment of suramin(II): interaction of suramin with phospholipids of various chain lengths. | |
| | |
MedLine Citation:
|
PMID: 8537885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previously, we reported that the entrapment of suramin in dipalmitoylphosphatidylcholine (DPPC, C16) multilamellar liposomes ranged from 25% to 65% and the addition of 30-50 mol% cholesterol (CHL) greatly reduced entrapment. Entrapment of small molecules similar to suramin, disodium 1,5-naphthalenedisulfonic acid (5.5%) and sodium 3-amino-2,7-naphthalenedisulfonic acid (1.2%), were very low. In the present study, the entrapment and interaction of suramin with dilauroylphosphatidylcholine (DLPC, C12), dimyristoylphosphatidylcholine (DMPC, C14), and distearoylphosphatidylcholine (DSPC, C18) liposomes was investigated. DLPC and DMPC showed 2-3-fold higher entrapment percentages (95.1% and 74.2%, respectively) than DPPC (37%). However, the entrapment with DSPC (29%) was about 25% lower than DPPC. Adding 50 mol% cholesterol greatly reduced suramin entrapment for all phospholipids. The entrapment of polysulfonated dyes such as Evans blue, Direct blue 1, or Trypan blue, which are structurally similar to suramin, was found to be in the same order of DLPC > DMPC > DPPC > DSPC. Differential scanning calorimetry of aqueous dispersions of DLPC and DMPC with suramin showed more apparent interaction than for DPPC and DSPC. These results suggest that a large portion of the associated suramin and other polysulfonated compounds results from binding to the surface of the phospholipid bilayer or intercalation into the liposomal bilayer. The phospholipid chain length effect on entrapment may be due to the lower net van der Waals interaction between hydrocarbon chains for shorter acyl chains which also increases the bilayer intermolecular spacing. Such effects could then increase the ability of suramin to interact with individual phospholipid molecules. |
| | |
Authors:
|
H C Chang; D R Flanagan |
Related Documents
:
|
18971215 - Arachidonic acid increases antifungal susceptibility of candida albicans and candida du... 2128685 - Calcium-dependent phospholipid catabolism and arachidonic acid mobilization in cerebral... 6290805 - Age-dependent alterations in lipids and function of rat heart sarcolemma. 7462155 - Incorporation of leucine into phospholipids of bacteroides thetaiotaomicron. 17645925 - Interaction between amino acid neurotransmitters and opioid receptors in hyperthermia-i... 16414735 - Lipid components of caralluma edulis (edgew.) hook. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of pharmaceutical sciences Volume: 84 ISSN: 0022-3549 ISO Abbreviation: J Pharm Sci Publication Date: 1995 Sep |
Date Detail:
|
Created Date: 1996-02-05 Completed Date: 1996-02-05 Revised Date: 2006-04-17 |
Medline Journal Info:
|
Nlm Unique ID: 2985195R Medline TA: J Pharm Sci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1078-82 Citation Subset: IM |
Affiliation:
|
Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City 52242, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Azo Compounds
/
chemistry Calorimetry, Differential Scanning Coloring Agents Evans Blue Liposomes Membranes, Artificial Phospholipids Suramin / administration & dosage*, chemistry Temperature Trypan Blue |
| Chemical | |
Reg. No./Substance:
|
0/Azo Compounds; 0/Coloring Agents; 0/Liposomes; 0/Membranes, Artificial; 0/Phospholipids; 145-63-1/Suramin; 2610-05-1/pontamine sky blue; 314-13-6/Evans Blue; 72-57-1/Trypan Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Water mobility in poly(ethylene glycol)-, poly(vinylpyrrolidone)-, and gelatin-water systems, as ind...
Next Document: A new pH-metric methodology for the determination of thermodynamic inclusion constants of guest/cycl...