| Liposomal VIP attenuates phenylephrine- and ANG II-induced vasoconstriction in vivo. | |
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MedLine Citation:
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PMID: 9688697 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to determine whether vasoactive intestinal peptide (VIP) modulates vasoconstriction elicited by phenylephrine and ANG II in vivo and, if so, to begin to elucidate the mechanisms underlying this phenomenon. Using intravital microscopy, we found that suffusion of phenylephrine and ANG II elicits significant vasoconstriction in the in situ hamster cheek pouch that is potentiated by VIP-(10-28), a VIP receptor antagonist, but not by VIP-(1-12) (P < 0.05). Aqueous VIP has no significant effects on phenylephrine- and ANG II-induced vasoconstriction. However, VIP on sterically stabilized liposomes (SSL), a formulation where VIP assumes a predominantly alpha-helix conformation, significantly attenuates this response. Maximal effect is observed within 30 min and is no longer seen after 60 min. Empty SSL are inactive. Indomethacin has no significant effects on responses induced by VIP on SSL. The vasodilators ACh, nitroglycerin, calcium ionophore A-23187, 8-bromo-cAMP, and isoproterenol have no significant effects on phenylephrine- and ANG II-induced vasoconstriction. Collectively, these data suggest that vasoconstriction modulates VIP release in the in situ hamster cheek pouch and that alpha-helix VIP opposes alpha-adrenergic- and ANG II-induced vasoconstriction in this organ in a reversible, prostaglandin-, NO-, cGMP-, and cAMP-independent fashion. |
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Authors:
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H Ikezaki; H Onyüksel; I Rubinstein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 275 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-09-16 Completed Date: 1998-09-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R588-95 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Angiotensin II / antagonists & inhibitors, pharmacology* Animals Arterioles / drug effects, physiology* Calcimycin / pharmacology Cheek Cricetinae Cyclic GMP / analogs & derivatives, pharmacology Drug Carriers Indomethacin / pharmacology Isoproterenol / pharmacology Liposomes Male Mesocricetus Mouth Mucosa / blood supply Nitroglycerin / pharmacology Phenylephrine / antagonists & inhibitors, pharmacology* Protein Structure, Secondary Vasoactive Intestinal Peptide / administration & dosage, pharmacology*, physiology Vasoconstriction / drug effects, physiology* Vasodilator Agents / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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DE-00386/DE/NIDCR NIH HHS; DE-10347/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Liposomes; 0/Vasodilator Agents; 11128-99-7/Angiotensin II; 31356-94-2/8-bromocyclic GMP; 37221-79-7/Vasoactive Intestinal Peptide; 51-84-3/Acetylcholine; 52665-69-7/Calcimycin; 53-86-1/Indomethacin; 55-63-0/Nitroglycerin; 59-42-7/Phenylephrine; 7665-99-8/Cyclic GMP; 7683-59-2/Isoproterenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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