Document Detail


Lipoproteins in Drosophila melanogaster--assembly, function, and influence on tissue lipid composition.
MedLine Citation:
PMID:  22844248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interorgan lipid transport occurs via lipoproteins, and altered lipoprotein levels correlate with metabolic disease. However, precisely how lipoproteins affect tissue lipid composition has not been comprehensively analyzed. Here, we identify the major lipoproteins of Drosophila melanogaster and use genetics and mass spectrometry to study their assembly, interorgan trafficking, and influence on tissue lipids. The apoB-family lipoprotein Lipophorin (Lpp) is the major hemolymph lipid carrier. It is produced as a phospholipid-rich particle by the fat body, and its secretion requires Microsomal Triglyceride Transfer Protein (MTP). Lpp acquires sterols and most diacylglycerol (DAG) at the gut via Lipid Transfer Particle (LTP), another fat body-derived apoB-family lipoprotein. The gut, like the fat body, is a lipogenic organ, incorporating both de novo-synthesized and dietary fatty acids into DAG for export. We identify distinct requirements for LTP and Lpp-dependent lipid mobilization in contributing to the neutral and polar lipid composition of the brain and wing imaginal disc. These studies define major routes of interorgan lipid transport in Drosophila and uncover surprising tissue-specific differences in lipoprotein lipid utilization.
Authors:
Wilhelm Palm; Julio L Sampaio; Marko Brankatschk; Maria Carvalho; Ali Mahmoud; Andrej Shevchenko; Suzanne Eaton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-26
Journal Detail:
Title:  PLoS genetics     Volume:  8     ISSN:  1553-7404     ISO Abbreviation:  PLoS Genet.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-30     Completed Date:  2013-01-04     Revised Date:  2013-03-14    
Medline Journal Info:
Nlm Unique ID:  101239074     Medline TA:  PLoS Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1002828     Citation Subset:  IM    
Affiliation:
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apolipoproteins B / metabolism
Biological Transport / genetics*
Carrier Proteins / genetics,  metabolism
Diglycerides / metabolism
Drosophila Proteins / metabolism
Drosophila melanogaster* / genetics,  metabolism
Fat Body / metabolism
Fatty Acids / metabolism
Hemolymph / metabolism
Imaginal Discs / metabolism
Lipid Metabolism / genetics*
Lipoproteins* / chemistry,  genetics,  metabolism
Tissue Distribution
Chemical
Reg. No./Substance:
0/Apolipoproteins B; 0/Carrier Proteins; 0/Cv-d protein, Drosophila; 0/Diglycerides; 0/Drosophila Proteins; 0/Fatty Acids; 0/Lipoproteins; 0/lipid transfer protein; 0/lipophorin; 0/microsomal triglyceride transfer protein
Comments/Corrections

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