Document Detail

Lipoproteins are inhibitors of endothelium-dependent relaxation of rabbit aorta.
MedLine Citation:
PMID:  2105664     Owner:  NLM     Status:  MEDLINE    
The present study was performed to investigate plasma inhibitors of endothelium-dependent relaxation other than hemoglobin and low-density lipoprotein (LDL). We purified an inhibitor that contained a protein of 28,000 Da from human plasma by ammonium sulfate precipitation and serial chromatography. NH2-terminal sequence analysis revealed the protein to be homologous with human apolipoprotein A-I (Apo A-I), a major apolipoprotein of high-density lipoprotein (HDL). Very low-density lipoprotein (VLDL), LDL, and HDL obtained from rabbit plasma reversed endothelium-dependent relaxation of rabbit aorta induced by acetylcholine (ACh) and A23187 but did not inhibit relaxations induced by nitroglycerin or nitric oxide. These inhibitory activities were lost by delipidation of lipoproteins, and there were no differences in the inhibitory activity among these three lipoproteins on the basis of phospholipid concentration. Moreover, phospholipids such as phosphatidylcholine, phosphatidylinositol, and sphingomyelin reversed relaxations by ACh and A23187. Thus all lipoproteins inhibit endothelium-dependent relaxation, and this nonspecific inhibition seems to be due to the inhibition of production or release of endothelium-derived relaxing factor by phospholipids in the lipoprotein complex.
M Takahashi; Y Yui; H Yasumoto; T Aoyama; H Morishita; R Hattori; C Kawai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  258     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-05     Completed Date:  1990-03-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1-8     Citation Subset:  IM    
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
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MeSH Terms
Aorta / physiology*
Blood Physiological Phenomena
Endothelium, Vascular / physiology*
Lipoproteins / isolation & purification,  physiology*
Lipoproteins, HDL / pharmacology
Lipoproteins, LDL / pharmacology
Lipoproteins, VLDL / pharmacology
Nitric Oxide / pharmacology
Nitroglycerin / pharmacology
Phospholipids / pharmacology
Vasodilation / drug effects,  physiology*
Reg. No./Substance:
0/Lipoproteins; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 0/Phospholipids; 10102-43-9/Nitric Oxide; 55-63-0/Nitroglycerin

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