| Lipoprotein separation in a novel iodixanol density gradient, for composition, density, and phenotype analysis. | |
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MedLine Citation:
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PMID: 18337616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Separation of lipoproteins by traditional sequential salt density floatation is a prolonged process ( approximately 72 h) with variable recovery, whereas iodixanol-based, self-generating density gradients provide a rapid ( approximately 4 h) alternative. A novel, three-layered iodixanol gradient was evaluated for its ability to separate lipoprotein fractions in 63 subjects with varying degrees of dyslipidemia. Lipoprotein cholesterol, triglycerides, and apolipoproteins were measured in 21 successive iodixanol density fractions. Iodixanol fractionation was compared with sequential floatation ultracentrifugation. Iodixanol gradient formation showed a coefficient of variation of 0.29% and total lipid recovery from the gradient of 95.4% for cholesterol and 84.7% for triglyceride. Recoveries for VLDL-, LDL-, and HDL-cholesterol, triglycerides, and apolipoproteins were approximately 10% higher with iodixanol compared with sequential floatation. The iodixanol gradient effectively discriminated classic lipoproteins and their subfractions, and there was evidence for improved resolution of lipoproteins with the iodixanol gradient. LDL particles subfractionated by the gradient showed good correlation between density and particle size with small, dense LDL (<25.5 nm) separated in fractions with density >1.028 g/dl. The new iodixanol density gradient enabled rapid separation with improved resolution and recovery of all lipoproteins and their subfractions, providing important information with regard to LDL phenotype from a single centrifugation step with minimal in-vitro modification of lipoproteins. |
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Authors:
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Michael S Yee; Darrell V Pavitt; Tira Tan; Soundararajan Venkatesan; Ian F Godsland; William Richmond; Desmond G Johnston |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-12 |
Journal Detail:
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Title: Journal of lipid research Volume: 49 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-19 Completed Date: 2008-08-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 1364-71 Citation Subset: IM |
Affiliation:
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Section of Endocrinology and Metabolic Medicine, Faculty of Medicine, Imperial College London, St Mary's Hospital, London W2 1NY, United Kingdom. m.yee@imperial.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Electrophoresis, Polyacrylamide Gel Humans Lipoproteins / chemistry, isolation & purification* Particle Size Phenotype Reproducibility of Results Triiodobenzoic Acids / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins; 0/Triiodobenzoic Acids; 92339-11-2/iodixanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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