| Lipoprotein lipase enables triacylglycerol hydrolysis by perfused newborn rat liver. | |
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MedLine Citation:
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PMID: 1928350 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fasted 1-day-old rat liver has high heparin-releasable (endothelial) lipoprotein lipase (LPL) activity, and its hepatocytes synthesize LPL protein. To test the physiological role of this LPL, we perfused the isolated organ with a 0.8 mM triacylglycerol (TAG) (Intralipid + glycerol tri[3H]oleate) 6.3% serum medium. Samples of the recirculated perfusate were taken at different times to determine 3H in TAG, free fatty acid (FFA), and water-soluble (WS) fractions. In the medium [3H]TAG disappeared and [3H]FFA and [3H]WS fractions appeared linearly with time. This TAG hydrolysis was 1) absent when medium was recirculated without liver, 2) not affected by chloroquine addition, 3) inhibited by anti-LPL immunoglobulins, 4) absent when serum was omitted from the medium, and 5) restituted when apolipoprotein CII was added to the medium without serum. Therefore, lysosomal lipase is not involved in this TAG hydrolysis, the features of which are characteristic of LPL, not of the so-called "hepatic endothelial lipase." Thus LPL activity enables the neonatal rat liver to hydrolyze and take up circulating TAG, i.e., has the same function as extrahepatic LPL. |
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Authors:
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L Giménez-Llort; J Vilanova; N Skottova; G Bengtsson-Olivecrona; M Llobera; M Q Robert |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 261 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-11-18 Completed Date: 1991-11-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G641-7 Citation Subset: IM |
Affiliation:
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Department de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Apolipoprotein C-II Apolipoproteins C / pharmacology Blood Physiological Phenomena Chloroquine / pharmacology Hydrolysis / drug effects Immunoglobulin G / immunology Lipoprotein Lipase / immunology, physiology* Liver / metabolism* Perfusion Rats Rats, Inbred Strains Triglycerides / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein C-II; 0/Apolipoproteins C; 0/Immunoglobulin G; 0/Triglycerides; 54-05-7/Chloroquine; EC 3.1.1.34/Lipoprotein Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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