| Lipoprotein enrichment in orange insoluble particulate matter reproducibly appearing in cryoprecipitate. | |
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MedLine Citation:
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PMID: 21488882 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background and Objective Cryoprecipitate prepared from two whole blood donations from the same donor contained insoluble orange particulate material (OPM). We sought to identify the OPM. Materials and Methods OPM was recovered from the blood product by centrifugation, dissolved in sodium dodecyl sulphate (SDS) and analysed by SDS-polyacrylamide gel electrophoresis and immunoblotting. Results Solubilized OPM was enriched in apolipoproteins B and E, but not apolipoprotein A1, immunoglobulin G or albumin, suggesting lipoprotein enrichment in OPM. Subsequent clinical laboratory blood tests confirmed low-density lipoprotein hyperlipidaemia with normal triglyceride levels. Further, cryoprecipitate production from this donor was prevented by implementation of national predominantly male plasma policies. Conclusion Cryoprecipitate produced from hyperlipidaemic donors may contain insoluble particles that render it inappropriate for transfusion. |
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Authors:
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W P Sheffield; V Bhakta; B Trigatti; C Jenkins |
Publication Detail:
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Type: Journal Article Date: 2010-11-23 |
Journal Detail:
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Title: Vox sanguinis Volume: 100 ISSN: 1423-0410 ISO Abbreviation: Vox Sang. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413606 Medline TA: Vox Sang Country: England |
Other Details:
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Languages: eng Pagination: 422-5 Citation Subset: IM |
Copyright Information:
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© 2010 The Author(s). Vox Sanguinis © 2010 International Society of Blood Transfusion. |
Affiliation:
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Canadian Blood Services Research and Development, Ottawa, ON, Canada Quality Monitoring Program, Ottawa, ON, Canada Departments of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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