Document Detail


Lipopolysaccharides from Mesorhizobium huakuii and Mesorhizobium ciceri: chemical and immunological comparative data.
MedLine Citation:
PMID:  12545212     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipopolysaccharides of two Mesorhizobium species of different host specificity were compared: M. huakuii and M. ciceri. M. huakuii sp. was represented by five strains with special consideration of M. huakuii IFO 15243(T). SDS/PAGE profiles revealed that all M. huakuii LPS preparations contained low molecular mass fractions (LPS-II) of the same molecular size. All of lipopolysaccharides contained high molecular mass fractions (LPS-I). However, the high molecular mass fraction from each strain possessed an individual molecular size distribution pattern. The crossreactivity of blotted lipopolysaccharides with rabbit polyclonal antibodies against Mesorhizobium huakuii IFO 15243(T) whole bacteria indicated the presence of common epitope(s) within the investigated Mesorhizobium huakuii strains. Moreover, LPS from M. huakuii S52 also reacted with anti M. ciceri HAMBI 1750 serum showing that there are epitopes common for different mesorhizobial species. LPS isolated from Mesorhizobium huakuii strain IFO 15243(T) contained neutral sugars: L-6-deoxytalose, L-rhamnose, D-galactose and D-glucose, aminosugars:D-quinovosamine, D-glucosamine, D-2,3-diamino-2,3-dideoxyglucose and D-galacturonic and D-glucuronic acids. In the LPS preparation, fatty acids typical for Mesorhizobium strains were detected. 3-Hydroxydodecanoic, 3-hydroxy-iso-tridecanoic, 3-hydroxyeicosanoic, 3-hydroxyheneicosanoic and 3-hydroxydocosenoic acids were the major amide linked fatty acids, while iso -heptadecanoic, eicosanoic, docosenoic, as well as 27-hydroxyoctacosanoic and 27-oxooctacosanoic acids were the dominant ester linked fatty residues.
Authors:
Adam Choma
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  49     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  2002  
Date Detail:
Created Date:  2003-01-24     Completed Date:  2004-01-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  1043-52     Citation Subset:  IM    
Affiliation:
Department of General Microbiology, Maria Curie-Skłodowska University, Lublin, Poland. achoma@biotop.umcs.lublin.pl
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MeSH Terms
Descriptor/Qualifier:
Alphaproteobacteria / chemistry*,  classification*,  immunology
Animals
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Immune Sera / immunology
Lipopolysaccharides / chemistry*,  immunology*
Rabbits
Species Specificity
Chemical
Reg. No./Substance:
0/Immune Sera; 0/Lipopolysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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