| Lipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamate. | |
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MedLine Citation:
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PMID: 21081156 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Interleukin-10 (IL-10) is a cytokine with important endogenous and therapeutic anti-inflammatory effects. Given this, it is of interest to investigate factors that modulate IL-10 levels in the central nervous system. IL-10 is released after lipopolysaccharide (LPS) stimulation of microglia. Microglia also express functional glutamate receptors and in inflammatory conditions are exposed to increased levels of glutamate. The aim of this research, then, is to investigate whether glutamate can modulate lipopolysaccharide stimulation of IL-10 release from neonatal rat spinal cord microglia. Enzyme-linked immunosorbent assays (ELISAs) were used to quantify IL-10 release from cultured neonatal spinal cord microglia and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure IL-10 mRNA expression. Glutamate (1 mM) significantly increased LPS (1 μg/ml)-stimulated IL-10 release from microglia by 172% (EC(50) of 103 μM) and significantly upregulated IL-10 mRNA levels. Glutamate potentiated LPS-stimulated IL-10 release by binding all subtypes of glutamate receptor. These results show that glutamate substantially increases the release of an anti-inflammatory cytokine from neonatal spinal cord microglia activated by a high concentration of LPS. |
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Authors:
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E L Werry; G J Liu; M D Lovelace; R Nagarajah; I B Hickie; M R Bennett |
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Publication Detail:
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Type: Journal Article Date: 2010-11-21 |
Journal Detail:
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Title: Neuroscience Volume: 175 ISSN: 1873-7544 ISO Abbreviation: Neuroscience Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: United States |
Other Details:
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Languages: eng Pagination: 93-103 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 IBRO. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Brain and Mind Research Institute and Bosch Institute, The University of Sydney, NSW, 2006, Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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