Document Detail

Lipopolysaccharide-induced lung injury in mice. II. Evaluation of functional damage in isolated parenchyma strips.
MedLine Citation:
PMID:  10799284     Owner:  NLM     Status:  MEDLINE    
Pulmonary inflammatory diseases are characterized by changes in airway responsiveness. This phenomenon is commonly related to the action of inflammatory mediators produced by infiltrated leukocytes. The aim of this study was to investigate in an ex vivo experimental model the effect of acute instillation of lipopolysaccharide (bacterial endotoxin; LPS) on lung parenchyma contractility. We firstly characterized the responsiveness of isolated murine lung to airway stimuli. Murine parenchymal strips were found to be mainly sensitive to 5-hydroxytryptamine (5-HT) while the cholinergic agonist, methacholine (MCh), evoked a smaller contractile response. 5-HT responsiveness was inhibited by methysergide. No significant parenchymal contraction was evoked by histamine, substance P and bradykinin. Lung responsiveness to 5-HT was significantly reduced by in vivo LPS treatment and this effect was only partially paralleled by leukocyte infiltration. In addition, LPS-induced hyporesponsiveness was significantly inhibited by betamethasone (BMS) or pentoxifylline (PTX) pretreatment suggesting that 5-HT lung hyporesponsiveness could be mediated by LPS-induced inflammatory mediators such as inflammatory cytokines.
L Brandolini; C Asti; V Ruggieri; A Intilangelo; L Pellegrini; R Chiusaroli; G F Caselli; R Bertini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pulmonary pharmacology & therapeutics     Volume:  13     ISSN:  1094-5539     ISO Abbreviation:  Pulm Pharmacol Ther     Publication Date:  2000  
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9715279     Medline TA:  Pulm Pharmacol Ther     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  71-8     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Section of Pharmacology, DOMPE S.p.A., L'Aquila, Italy.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology
Betamethasone / pharmacology
Bradykinin / pharmacology
Dose-Response Relationship, Drug
Drug Interactions
Enzyme Inhibitors / pharmacology
Histamine / pharmacology
Lipopolysaccharides / toxicity*
Lung Diseases / chemically induced*,  physiopathology
Methacholine Chloride / pharmacology
Muscle Contraction / drug effects
Pentoxifylline / pharmacology
Serotonin / pharmacology
Substance P / pharmacology
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Enzyme Inhibitors; 0/Lipopolysaccharides; 33507-63-0/Substance P; 378-44-9/Betamethasone; 50-67-9/Serotonin; 51-45-6/Histamine; 58-82-2/Bradykinin; 62-51-1/Methacholine Chloride; 6493-05-6/Pentoxifylline

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