Document Detail


Lipopolysaccharide-induced activation of coagulation in neonatal cord and adult blood monitored by thrombelastography.
MedLine Citation:
PMID:  19477486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Although precisely balanced hemostasis in newborns is rapidly changing during early development. During gram-negative sepsis, lipopolysaccharide (LPS) activates toll-like receptor (TLR) 4 and induces complex responses of immune system and hemostasis. In the present study we compared LPS-induced activation of coagulation in cord blood (CB) samples of healthy newborns to whole blood (WB) samples from healthy adult volunteers. MATERIALS AND METHODS: Samples were incubated with LPS for various incubation periods (0-2-3-4 hrs), and coagulation was measured by rotation thrombelastography (TEG). RESULTS: All parameters (clotting time (CT), clot formation time (CFT), maximal clot formation (MCF), and angle alpha) were affected with increasing incubation period with LPS (100 ng/ml) in adult and cord WB. In the absence of LPS, CTs and CFTs were significantly shorter and MCFs and angels alpha were significantly longer in cord than in adult WB samples. Reduction of CT due to LPS incubation was significantly stronger in CB than in adult WB samples. Pyrrolidine dithiocarbamate, a specific inhibitor of the NFkappaB pathway, inhibited the effects of LPS on CT in adult and cord WB. CONCLUSION: In summary, TEG proved to be a sensitive and reliable tool for the determination of LPS-induced tissue factor mediated activation of hemostasis in whole blood samples from adults and neonates.
Authors:
Lutz Koch; Stefan Hofer; Markus A Weigand; David Frommhold; Johannes Poeschl
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Publication Detail:
Type:  Journal Article     Date:  2009-05-27
Journal Detail:
Title:  Thrombosis research     Volume:  124     ISSN:  1879-2472     ISO Abbreviation:  Thromb. Res.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  463-7     Citation Subset:  IM    
Affiliation:
Division of Neonatology, Department of Paediatrics, University of Heidelberg Medical School, Im Neuenheimer Feld 430, Heidelberg, Germany. lutz.koch@med.uni-heidelberg.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Blood Coagulation / drug effects*
Fetal Blood / metabolism*
Humans
Infant, Newborn
Lipopolysaccharides / pharmacology*
Reference Values
Reproducibility of Results
Thrombelastography / methods*
Whole Blood Coagulation Time
Chemical
Reg. No./Substance:
0/Lipopolysaccharides

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