| Lipopolysaccharide effects on the proliferation of NRK52E cells via alternations in gap-junction function. | |
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MedLine Citation:
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PMID: 22743374 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: Gap junctions regulate proper kidney function by facilitating intercellular communication, vascular conduction, and tubular purinergic signaling. However, no clear relationship has been described between gap-junction function and acute kidney injury induced by the endotoxin lipopolysaccharide (LPS). METHODS: Normal rat kidney epithelial cells (NRK52E cells) were seeded at high and low densities to promote or impede gap-junction formation, respectively, and establish distinctive levels of intercellular communication in culture. Cells were then challenged with LPS at various concentrations (10-1,000 ng/mL). LPS-induced formation and function of gap junctions were assessed by measuring changes in cell proliferation and colony-forming rates, fluorescent dye transmission to adjacent cells, expression levels of connexin43, and repositioning of confluent cells in response to the gap junction inhibitor oleamide or agonist retinoic acid. RESULTS: The cell proliferation rate and colony-forming rate of high- and low-density NRK52E cells were decreased upon LPS challenge, in a dose-dependent manner. The colony-forming rate of confluent high-density cells was significantly lower than that of low-density cells. Oleamide treatment raised the LPS-induced colony-forming rate of high-density cells, whereas retinoic acid decreased the rate. Neither oleamide nor retinoic acid significantly affected the LPS-induced colony-forming rate of low-density cells. Fluorescence transmission of high-density cells was reduced by LPS challenge, in a dose-dependent manner, but inclusion of retinoic acid increased the LPS-induced transmission of fluorescence. LPS challenge of either high- or low-density NRK52E cells resulted in down-regulated connexin43 expression. CONCLUSION: Gap-junction function plays an important role in concentration-dependent cytotoxic effect of LPS on normal rat kidney cells in vitro. |
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Authors:
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Ziqing Hei; Ailan Zhang; Jing Wei; Xiaoliang Gan; Yanling Wang; Gangjian Luo; Xiaoyun Li |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of trauma and acute care surgery Volume: 73 ISSN: 2163-0763 ISO Abbreviation: J Trauma Acute Care Surg Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101570622 Medline TA: J Trauma Acute Care Surg Country: United States |
Other Details:
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Languages: eng Pagination: 67-72 Citation Subset: AIM; IM |
Affiliation:
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From the Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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