| Lipopolysaccharide disrupts the directional persistence of alveolar myofibroblast migration through EGF receptor. | |
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MedLine Citation:
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PMID: 22245996 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Bronchopulmonary dysplasia (BPD) is characterized by alveolar simplification with decreased alveolar number and increased airspace size. Formation of alveoli involves a process known as secondary septation triggered by myofibroblasts. This study investigated the underlying mechanisms of altered lung morphogenesis in a rat model of BPD induced by intra-amniotic injection of lipopolysaccharide (LPS). Results showed that LPS disrupted alveolar morphology and led to abnormal localization of myofibroblasts in the lung of newborn rats, mostly in primary septa with few in secondary septa. To identify potential mechanisms, in vitro experiments were carried out to observe the migration behavior of myofibroblasts. The migration speed of lung myofibroblasts increased with LPS treatment, while the directional persistence decreased. We found that LPS induced activation of EGFR and over-expression of its ligand, TGF-α in myofibroblasts. AG1478, an EGFR inhibitor, abrogated the enhanced locomotivity of myofibroblasts by LPS, and also increased the directional persistence of myofibroblasts migration. Myofibroblasts showed a high asymmetry of phospho-EGFR localization which was absent after LPS treatment. Application of rhTGF- α to myofibroblasts decreased the directional persistence. Our findings indicated that asymmetry of phospho-EGFR localization in myofibroblasts was important for cell migration and its directional persistence. We speculate that LPS exposure disrupts the asymmetric localization of phospho-EGFR, leading to decreased stability of cell polarity and final abnormal location of myofibroblasts in vivo, which is critical to secondary septation, and may contribute to the arrested alveolar development in BPD. |
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Authors:
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Huiping Li; Xiaobing Yuan; Jun Tang; Yongjun Zhang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-13 |
Journal Detail:
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Title: American journal of physiology. Lung cellular and molecular physiology Volume: - ISSN: 1522-1504 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901229 Medline TA: Am J Physiol Lung Cell Mol Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1XinHua Hospital, Shanghai Institute for Pediatric Research, Shanghai Jiaotong University School of Medicine. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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