| Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4 pathway and perturbs oocyte meiotic progression in vitro. | |
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MedLine Citation:
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PMID: 21990308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infections of the reproductive tract or mammary gland with Gram-negative bacteria perturb ovarian function, follicular growth, and fecundity in cattle. We hypothesized that lipopolysaccharide (LPS) from Gram-negative bacteria stimulates an inflammatory response by ovarian granulosa cells that is mediated by Toll-like receptor (TLR) 4. The present study tested the capacity of bovine ovarian granulosa cells to initiate an inflammatory response to pathogen-associated molecular patterns and determined subsequent effects on the in vitro maturation of oocytes. Granulosa cells elicited an inflammatory response to pathogen-associated molecular patterns (LPS, lipoteichoic acid, peptidoglycan, or Pam3CSK4) with accumulation of the cytokine IL-6, and the chemokine IL-8, in a time- and dose-dependent manner. Granulosa cells responded acutely to LPS with rapid phosphorylation of TLR signaling components, p38 and ERK, and increased expression of IL6 and IL8 mRNA, although nuclear translocation of p65 was not evident. Targeting TLR4 with small interfering RNA attenuated granulosa cell accumulation of IL-6 in response to LPS. Endocrine function of granulosa cells is regulated by FSH, but here, FSH also enhanced responsiveness to LPS, increasing IL-6 and IL-8 accumulation. Furthermore, LPS stimulated IL-6 secretion and expansion by cumulus-oocyte complexes and increased rates of meiotic arrest and germinal vesicle breakdown failure. In conclusion, bovine granulosa cells initiate an innate immune response to LPS via the TLR4 pathway, leading to inflammation and to perturbation of meiotic competence. |
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Authors:
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John J Bromfield; I Martin Sheldon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-10-11 |
Journal Detail:
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Title: Endocrinology Volume: 152 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-25 Completed Date: 2012-02-16 Revised Date: 2012-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 5029-40 Citation Subset: AIM; IM |
Affiliation:
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Institute of Life Science, School of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, United Kingdom. j.bromfield@swansea.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Endocrine System / immunology Female Granulosa Cells / pathology* Inflammation / chemically induced* Interleukin-6 / secretion Lipopolysaccharides / pharmacology* Meiosis / drug effects* Oocytes / cytology* Signal Transduction / drug effects Toll-Like Receptor 4 / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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BB/F005121/1//Biotechnology and Biological Sciences Research Council; F005121/1//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6; 0/Lipopolysaccharides; 0/Toll-Like Receptor 4 |
| Comments/Corrections | |
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