| Lipoplex formation using liposomes prepared by ethanol injection. | |
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MedLine Citation:
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PMID: 20072896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cationic liposomes composed of 3beta-[N-(N'N'-dimethylaminoethane)carbamoyl] cholesterol (DC-Chol) and dioleoylphosphatidylethanolamine (DOPE) (DC-Chol/DOPE liposome, molar ratio, 1:1 or 3:2) prepared by the dry-film method have been often used as non-viral gene delivery vectors. We have shown that a more efficient transfection in medium with serum was achieved using DC-Chol/DOPE liposomes (molar ratio, 1:2) than those (3:2), and preparation method by a modified ethanol injection than the dry-film. The most efficient DC-Chol/DOPE liposome for gene transfer was molar ratio (1:2) and prepared by a modified ethanol injection method. The enhanced transfection is related to an increase in the release of DNA in the cytoplasm by the large lipoplex during incubation in opti-MEM I reduced-serum medium (optiMEM), not to an increased cellular association with the lipoplex. Cationic liposomes rich in DOPE prepared by a modified ethanol injection method will help to improve the efficacy of liposome vector systems for gene delivery. |
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Authors:
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Yoshie Maitani |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 605 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 393-403 Citation Subset: IM |
Affiliation:
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Institute of Medicinal Chemistry, Hoshi University, Shinagawa, Tokyo, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Cations
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chemistry Cell Line, Tumor Cholesterol / analogs & derivatives*, chemistry DNA / administration & dosage* Ethanol Genes, Reporter Hela Cells Humans Liposomes / chemistry* Luciferases / genetics Phosphatidylethanolamines / chemistry* Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Liposomes; 0/Phosphatidylethanolamines; 137056-72-5/3-(N-(N',N'-dimethylaminoethane)carbamoyl)cholesterol; 2462-63-7/dioleoyl phosphatidylethanolamine; 57-88-5/Cholesterol; 64-17-5/Ethanol; 9007-49-2/DNA; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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