Document Detail


Lipophosphoglycan-like glycoconjugate of Tritrichomonas foetus and Trichomonas vaginalis.
MedLine Citation:
PMID:  8433719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipophosphoglycan-like glycoconjugates were isolated, purified and partially characterized from Tritrichomonas foetus and Trichomonas vaginalis. Cell surface radiolabeling of both trichomonads by the galactose oxidase/NaB[3H]4 technique indicated that the glycoconjugate was located on the cell surface of the parasites. The glycoconjugates were extracted from the delipidated residue fraction with the solvent, water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017) and were purified to homogeneity by Sepharose CL-4B followed by octyl-Sepharose chromatography and methanol precipitation. The glycoconjugates migrated as broad bands upon SDS-PAGE. The T. foetus glycoconjugate contained large amounts of fucose along with some mannose, galactose, glucosamine and glucose and trace amounts of galactosamine and inositol. The T. vaginalis glycoconjugate appeared to contain large amounts of glucosamine and galactose along with some glucose, mannose and traces of galactosamine and inositol. The surface-labeled glycoconjugates from both parasites was found to be deaminated with nitrous acid and susceptible to phosphatidylinositol-specific phospholipase C, indicating the presence of a phospholipid anchor. Furthermore, these glycoconjugate were found to contain phosphate and were labile to hydrolysis by mild acid, strongly suggesting that the intact molecule is related to Leishmania lipophosphoglycans (LPG). The most striking and the unique features of these glycoconjugate molecules are the presence of large amounts of fucose in T. foetus and glucosamine in T. vaginalis along with the presence of galactosamine in both parasites. These results indicate that these glycoconjugates are new types of LPG-like molecules expressed on the trichomonad cell surface and are structurally distinct from Leishmania LPG.
Authors:
B N Singh
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  57     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-03-17     Completed Date:  1993-03-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  281-94     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, SUNY Health Science Center, Syracuse 13210.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbohydrate Sequence
Carbohydrates / analysis
Cell Membrane / metabolism
Glycoconjugates / chemistry,  isolation & purification,  metabolism*
Glycosphingolipids / chemistry,  isolation & purification,  metabolism*
Immunochemistry
Molecular Sequence Data
Molecular Structure
Trichomonas vaginalis / metabolism*
Tritrichomonas foetus / metabolism*
Grant Support
ID/Acronym/Agency:
AI-36680/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Glycoconjugates; 0/Glycosphingolipids; 0/lipophosphonoglycan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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