| Lipocalin 2-mediated growth suppression is evident in human erythroid and monocyte/macrophage lineage cells. | |
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MedLine Citation:
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PMID: 18064607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipocalin 2 (LCN2), a secreted protein of the lipocalin family, induces apoptosis in some types of cells and inhibits bacterial growth by sequestration of the iron-laden bacterial siderophore. We have recently reported that LCN2 inhibits the production of red blood cells in the mouse. Here we analyzed the role of LCN2 in human hematopoiesis. Expression of LCN2 was observed not only in mature cells such as those of the granulocyte/macrophage and erythroid lineages but also in hematopoietic stem/progenitor cells. We also examined expression of two candidate receptors for LCN2, brain type organic cation transporter (BOCT) and megalin, in various cell types. BOCT showed relatively high levels of expression in erythroid and hematopoietic stem/progenitor cells but lower levels in granulocyte/macrophage and T lymphoid cells. Megalin was expressed at high levels in T lymphoid and erythroid cells but at lower levels in granulocyte/macrophage lineage cells. LCN2 suppressed the growth of erythroid and monocyte/macrophage lineages in vitro, but did not have this effect on cells of other lineages. In addition, immature hematopoietic stem/progenitor cells were not sensitive to LCN2. These results demonstrate a lineage-specific role for LCN2 in human hematopoiesis that is reminiscent of its effects upon mouse hematopoiesis and strongly suggest an important in vivo function of LCN2 in the regulation of human hematopoiesis. |
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Authors:
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Kenichi Miharada; Takashi Hiroyama; Kazuhiro Sudo; Inaho Danjo; Toshiro Nagasawa; Yukio Nakamura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 215 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-02-27 Completed Date: 2008-03-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 526-37 Citation Subset: IM |
Copyright Information:
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(c) 2007 Wiley-Liss, Inc. |
Affiliation:
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Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acute-Phase Proteins
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metabolism,
pharmacology,
physiology* Animals Apoptosis Cell Division / drug effects, physiology Cell Lineage Cells, Cultured Erythroid Cells / cytology*, metabolism Female Granulocytes / metabolism Hematopoiesis / physiology Hematopoietic Stem Cells / cytology, metabolism, physiology Humans LDL-Receptor Related Protein 2 / metabolism Lipocalins / metabolism, pharmacology, physiology* Lymphocytes / metabolism Macrophages / cytology*, metabolism Mice Mice, Inbred C57BL Monocytes / cytology*, metabolism, physiology Organic Cation Transport Proteins / metabolism Proto-Oncogene Proteins / metabolism, pharmacology, physiology* Recombinant Proteins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acute-Phase Proteins; 0/LCN2 protein, human; 0/LDL-Receptor Related Protein 2; 0/Lipocalins; 0/Organic Cation Transport Proteins; 0/Proto-Oncogene Proteins; 0/Recombinant Proteins; 0/SLC22A17 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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