Document Detail


Lipoarabinomannan localization and abundance during growth of Mycobacterium smegmatis.
MedLine Citation:
PMID:  21840972     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipoarabinomannan (LAM) is a structurally heterogeneous amphipathic lipoglycan present in Mycobacterium spp. and other actinomycetes, which constitutes a major component of the cell wall and exhibits a wide spectrum of immunomodulatory effects. Analysis of Mycobacterium smegmatis subcellular fractions and spheroplasts showed that LAM and lipomannan (LM) were primarily found in a cell wall-enriched subcellular fraction and correlated with the presence (or absence) of the mycolic acids in spheroplast preparations, suggesting that LAM and LM are primarily associated with the putative outer membrane of mycobacteria. During the course of these studies significant changes in the LAM/LM content of the cell wall were noted relative to the age of the culture. The LAM content of the M. smegmatis cell wall was dramatically reduced as the bacilli approached stationary phase, whereas LM, mycolic acid, and arabinogalactan content appeared to be unchanged. In addition, cell morphology and acid-fast staining characteristics showed variations with growth phase of the bacteria. In the logarithmic phase, the bacteria were found to be classic rod-shaped acid-fast bacilli, while in the stationary phase M. smegmatis lost the characteristic rod shape and developed a punctate acid-fast staining pattern with carbolfuchsin. The number of viable bacteria was independent of LAM content and phenotype. Taken together, the results presented here suggest that LAM is primarily localized with the mycolic acids in the cell wall and that the cellular concentration of LAM in M. smegmatis is selectively modulated with the growth phase.
Authors:
Rakesh K Dhiman; Premkumar Dinadayala; Gavin J Ryan; Anne J Lenaerts; Alan R Schenkel; Dean C Crick
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-08-12
Journal Detail:
Title:  Journal of bacteriology     Volume:  193     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-28     Completed Date:  2011-11-15     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5802-9     Citation Subset:  IM    
Affiliation:
Mycobacteria Research Laboratories, Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA. dean.crick@colostate.edu
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Cell Wall / genetics,  metabolism*
Lipopolysaccharides / metabolism*
Mycobacterium smegmatis / genetics,  growth & development*,  metabolism*
Grant Support
ID/Acronym/Agency:
AI04915/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 0/lipoarabinomannan; 0/lipomannan
Comments/Corrections

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