Document Detail


Lipids of the zygomycete Absidia corymbifera F-965.
MedLine Citation:
PMID:  15184008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell lipids of the zygomycete Absidia corymbifera F-965 extracted with isopropanol and CHCl3-MeOH mixtures at the exponential growth phase comprise 20+/-2% of mycelium dry wt. The lipids consist of: triacylglycerols (51% of the total lipids extracted), diacylglycerols (9%), monoacylglycerols (3%), ergosterol (5%), ergosterol peroxide (5alpha,8alpha-epidioxyergosta-6,22-diene-3beta-ol) (3%), fatty-acid esters of ergosterol (less than 0.5%), free fatty acids (4%), glucocerebroside (3%), and glycerophospholipids (22%). The main phospholipids are phosphatidylethanolamine (39% of the total phospholipids), phosphatidyl-myo-inositol (17%), diphosphatidylglycerol (12%), phosphatidic acid (7%), phosphatidylcholine (6%), phosphatidylglycerol (3%), and two unusual phospholipids reported earlier, N-acetylphosphatidylethanolamine (7%) and N-ethoxycarbonyl phosphatidylethanolamine (9%). In addition, two unknown acidic phospholipids are present in traces. Saturated fatty acids of the lipids are dominated by n-hexadecanoic acid and unsaturated ones by octadecenoic acid; octadecadienoic and octadecatrienoic acids are present in lesser amounts. Ergosterol peroxide as well as the above glucocerebroside which contains 9-methylsphinga-4(E),9(E)-dienine have first been revealed in zygomycetes.
Authors:
Stanislav G Batrakov; Inessa V Konova; Vladimir I Sheichenko; Stanislav E Esipov; Lyudmila A Galanina; Larisa N Istratova; Yana E Sergeeva
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytochemistry     Volume:  65     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-06-08     Completed Date:  2004-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1239-46     Citation Subset:  IM    
Affiliation:
Russian Research Centre Hydrobios, Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow 129301, Russia.
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MeSH Terms
Descriptor/Qualifier:
Absidia / chemistry*,  genetics
Chromatography, Gas
Ergosterol / analogs & derivatives*,  chemistry,  isolation & purification
Fatty Acids / analysis,  chemistry
Glucosylceramides / chemistry,  isolation & purification
Lipids / chemistry*,  isolation & purification
Molecular Structure
Phospholipids / chemistry,  isolation & purification
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Glucosylceramides; 0/Lipids; 0/Phospholipids; 2061-64-5/ergosterol-5,8-peroxide; 57-87-4/Ergosterol

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