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Lipids for nucleic Acid delivery: synthesis and particle formation.
MedLine Citation:
PMID:  23070764     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Lipidic vesicles have been extensively studied for their capacity to condensate and deliver nucleic acids to the cells. Many different amphiphilic lipidic structures have been proposed, each of them bringing some advances in nonviral gene transfection. The ionic or neutral nature of the lipids induces tremendous differences in the behavior of the corresponding liposomes, from the complexation of nucleic acid to the delivery to the cell. An efficient delivery in vitro or in vivo also depends closely on the structure of the lipids and very often, efficient liposomes in vitro have been found useless for in vivo administration.We wish to describe in this chapter the chemical synthesis of two different lipids, one cationic and the other essentially neutral, and the formulation to obtain liposomes and DNA/liposome complexes. The different ways and tricks for the formulation of the two different structures are especially highlighted.
Authors:
Michel Bessodes; Nathalie Mignet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  948     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-84     Citation Subset:  IM    
Affiliation:
Unité de Pharmacologie Chimique et Génétique, CNRS, UMR 8151, Paris, France.
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