Document Detail

Lipids in biocalcification: contrasts and similarities between intimal and medial vascular calcification and bone by NMR.
MedLine Citation:
PMID:  22651923     Owner:  NLM     Status:  MEDLINE    
Pathomechanisms underlying vascular calcification biogenesis are still incompletely understood. Biomineral from human atherosclerotic intimal plaques; human, equine, and bovine medial vascular calcifications; and human and equine bone was released from collagenous organic matrix by sodium hydroxide/sodium hypochlorite digestion. Solid-state (13)C NMR of intimal plaque mineral shows signals from cholesterol/cholesteryl esters and fatty acids. In contrast, in mineral from pure medial calcifications and bone mineral, fatty acid signals predominate. Refluxing (chloroform/methanol) intimal plaque calcifications removes the cholesterylic but not the fatty acyl signals. The lipid composition of this refluxed mineral now closely resembles that of the medial and bone mineral, which is unchanged by reflux. Thus, intimal and medial vascular calcifications and bone mineral have in common a pool of occluded mineral-entrained fatty acyl-rich lipids. This population of fatty acid may contain methyl-branched fatty acids, possibly representing lipoprotein particle remnants. Cell signaling and mechanistic parallels between physiological (orthotopic) and pathological (ectopic) calcification are also reflected thus in the NMR spectroscopic fingerprints of mineral-associated and mineral-entrained lipids. Additionally the atherosclerotic plaque mineral alone shows a significant independent pool of cholesterylic lipids. Colocalization of mineral and lipid may be coincidental, but it could also reflect an essential mechanistic component of biomineralization.
David G Reid; Catherine M Shanahan; Melinda J Duer; Luis G Arroyo; Michael Schoppet; Roger A Brooks; Rachel C Murray
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-31
Journal Detail:
Title:  Journal of lipid research     Volume:  53     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2012-11-23     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1569-75     Citation Subset:  IM    
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MeSH Terms
Calcification, Physiologic*
Extracellular Matrix / metabolism
Lipid Metabolism*
Magnetic Resonance Spectroscopy
Solvents / chemistry
Tunica Intima / metabolism*
Vascular Calcification / metabolism*,  pathology
Grant Support
RG/11/14/29056//British Heart Foundation; //Biotechnology and Biological Sciences Research Council; //British Heart Foundation
Reg. No./Substance:

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