Document Detail

Lipids during Bufo arenarum oogenesis.
MedLine Citation:
PMID:  12828408     Owner:  NLM     Status:  MEDLINE    
The content and composition of phospholipids and triacylglycerols (TAGs) in Bufo arenarum oocytes in stages III and IV of their oogenesis were studied. The total amount of phospholipids in stage IV oocytes is 0.5-fold higher than in stage III oocytes. In both cases, the main phospholipids are phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A striking observation concerns the high level of diphosphatidylglycerol (DPG) in stage III oocytes, which could be indicative of a relatively larger mitochondrial population with respect to other oogenetic stages. A net increase in sphingomyelin content was found during oogenesis. This fact could be related to the role of this phospholipid in the signal transductional pathways. In PC, palmitic (16:0), linoleic (18:2) and oleic (18:1) are the major fatty acids for both types of oocytes, while in PE the main acyl groups are 18:1, 16:0, arachidonic acid (20:4n6) and 18:2. PE is more unsaturated than PC and both phospholipids are more unsaturated in stage III oocytes than in stage IV oocytes. The amount of triacylglycerols is 0.3-fold higher in stage IV oocytes than in stage III oocytes. In both stages, the main fatty acids are 18:2, 18:1 and 16:0. During oogenesis, a significant increase in 18:1 and 18:3n3, and a decrease in 18:2 of TAG were found. The unsaturation index of TAGs from stage IV oocytes is higher than that from stage III oocytes. The TAG increase during oogenesis is consistent with the putative use of these lipids as a source of energy in embryo development.
Ariana Bruzzone; Jorgelina Buschiazzo; Telma S Alonso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  11     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-06-27     Completed Date:  2004-02-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  95-100     Citation Subset:  IM    
Instituto de Investigaciones Bioquímicas de Bahía Blanca (UNS-CONICET), C.C. 857, B8000FWB Bahía Blanca, Argentina.
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MeSH Terms
Bufo arenarum
Cardiolipins / metabolism
Lipid Metabolism*
Oocytes / metabolism*
Oogenesis / physiology*
Phosphatidylcholines / metabolism
Sphingomyelins / metabolism
Reg. No./Substance:
0/Cardiolipins; 0/Phosphatidylcholines; 0/Sphingomyelins

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